作物学报Issue(9)：1324-1332,9.DOI:10.3724/SP.J.1006.2015.01324
苎麻肉桂酰辅酶A还原酶基因cDNA序列的克隆与分析
cDNA Cloning andAnalysis of Cinnamoyl-CoA Reductase Gene fromBoeh-meria nivea
摘要
Abstract
Two cDNA sequences of cinnamoyl-CoA reductase genes were cloned by RACE technology base on transcriptome sequencing data ofBoehmeria nivea, and their bioinformatics were analyzed. Their expression levels in tissues of phloem and xylem were also tested respectively in rapid-growth stage, maturation stage and late maturity stage by quantitative Real-time PCR. The results showed that theBnCCR1was 1056 bp in length and encoding 277 amino acids. Blast and protein structure analysis showed that its cDNA sequence shared a homology of 70% compared withBetula platyphylla andRicinus communis CCR, and its amino acids did a homology of 77% compared with that ofRicinus communis. Protein prediction showed its protein N terminal with a conservative field of 3 Beta-HSD/Epimerase/NAD-binding-10. The coding sequence ofBnCCR2gene was 1291 bp, and could be translated into a 248 amino acids. Blast and protein structure analysis showed that its cDNA sequence had a homology of 74% compared withPopulus trichocarpa CCR, and its amino acids had a homology of 81% compared with these ofRicinus communis andPopulus trichocarpa. Protein prediction showed its protein N terminal with a conservative field of 3 Beta-HSD/ Epimerase/NAD-binding-4. Three-dimensional structures ofBnCCR1 and BnCCR2had relatively high similarity with CCR genes inpetunias, and the similarity was 30.68% and 44.77% respectively. The result of qRT-PCR indicated that the expression of both BnCCR1 andBnCCR2 was different in different periods, while not in different tissues, but the levels of expression in different tissues showed significant differences. We speculated thatBnCCR1 and BnCCR2are different CCR genes in lignin metabolism of Boehmeria nivea, and the result of this study provide a theoretical basis for further exploration of their function in the ramie lignin biosynthesis and regulation.关键词
苎麻/肉桂酰辅酶A还原酶基因/木质素/组织表达Key words
Boehmeria nivea/Cinnamoyl-CoA reductase gene/Lignin/Tissue expression引用本文复制引用
唐映红,陈建荣,刘芳,袁有美,郭清泉,昌洪涛..苎麻肉桂酰辅酶A还原酶基因cDNA序列的克隆与分析[J].作物学报,2015,(9):1324-1332,9.基金项目
本研究由国家自然科学基金项目(31301374,31071457)资助。 （31301374,31071457）
