作物学报Issue(9)：1343-1352,10.DOI:10.3724/SP.J.1006.2015.01343
野生大豆碳酸盐胁迫应答基因GsMIPS2的克隆及功能分析
Cloning and Functional Analysis ofGlycine sojaBicarbonate StressResponsive GeneGsMIPS2
摘要
Abstract
To identify the key functional genes in response to bicarbonate stress inGlycine soja L. G07256, we constructed a gene expression profile under bicarbonate treatment using high throughput RNA-seq data, from which we selected a myo-inositol- 1-phosphate synthase gene whose expression was significantly induced by bicarbonate stress. By homology-based cloning, we acquired its full length cDNA and termed asGsMIPS2. The results of quantitative real-time PCR demonstrated the bicarbonate stress induced expression ofGsMIPS2 and its tissue expression specificity. We also verified the function ofGsMIPS2 in bicarbon-ate responses by using theGsMIPS2 transgenicArabidopsis combined with the T-DNA insertion lineatmips2. We revealed that the germination rate ofGsMIPS2 overexpression lines was significantly higher, while that ofatmips2 mutant line was much lower than that of wild type under bicarbonate stress. These results indicate the positive role ofGsMIPS2 in plant bicarbonate stress responses.关键词
野生大豆/GsMIPS2基因/肌醇-1-磷酸合成酶/碳酸盐胁迫Key words
Glycine soja/GsMIPS2/Myo-inositol-1-phosphate synthase/Bicarbonate stress引用本文复制引用
陈晨,孙晓丽,刘艾林,端木慧子,于洋,肖佳雷,朱延明..野生大豆碳酸盐胁迫应答基因GsMIPS2的克隆及功能分析[J].作物学报,2015,(9):1343-1352,10.基金项目
本研究由国家转基因生物新品种培育重大专项(2011ZX08004-002),国家自然科学基金项目(31171578),黑龙江省高校科技创新团队建设计划项目(2011TD055)和国家基础科学人才培养基金项目(J1210069)资助。 （2011ZX08004-002）
