作物学报Issue(9)：1372-1383,12.DOI:10.3724/SP.J.1006.2015.01372
大豆异黄酮及其组分含量的遗传分析与QTL检测
Genetic Analysis and QTL Mapping of Isoflavone Contents and Its Com-ponents in Soybean
摘要
Abstract
A set of 447 recombinant inbred lines (RILs) derived from the cross between cultivars Jingdou 23 (female parent) and Huibuzhi (semi-wild, male parent) was used to construct a new map. Isoflavone content and its components were quantitatively and qualitatively evaluated by using high performance liquid chromatography (HPLC). We analyzed inheritance and detected QTLs for isoflavone content and its components in soybean seeds using major gene plus polygene mixed inheritance analysis and WinQTLCart 2.5 composite interval mapping. The results showed that daidzin, daidzein, genistein, genistin, glycitin, and total isoflavone contents were controlled by four, four, two, three, two and two main-genes, respectively. However, polygene effects were not detected in the study. Forty-four quantitative trait loci (QTLs) for isoflavone contents and its components were mapped, including ten for daidzin, nine for genistein, four for daidzein, seven for glycitin, eight for genistin, and six for total isoflavone content. The stable QTLs related to daidzin, genistin, glycitin, isoflavone content were respectively detected to be located in the intervals of satt430–satt359, satt038–satt570, satt197–sat_128, and satt249–satt285 during two years, which could be used in marker-assisted selection (MAS) for soybean breeding.关键词
大豆/异黄酮含量/遗传分析/QTLKey words
Soybean/Isoflavone content/Genetic analysis/Quantitative trait loci引用本文复制引用
梁慧珍,余永亮,杨红旗,许兰杰,董薇,牛永光,张海洋,刘学义,方宣钧..大豆异黄酮及其组分含量的遗传分析与QTL检测[J].作物学报,2015,(9):1372-1383,12.基金项目
本研究由河南省科技创新杰出人才计划(114200510002),国家转基因生物新品种培育科技重大专项(2014ZX0800402B),河南省重点科技攻关计划项目(132102110091)和河南省农业科学院专项资金(201315603,201315615,201218334)资助。 （114200510002）
