中医药学报Issue(4)：33-36,4.
防己水煎液及其不同拆分部位对脂多糖诱导下 RAW264．7细胞炎症因子的影响
Effect of Inflammatory Cytokines in the LPS-induced RAW264.7 Cells by the Decoction and Its Split Components from Stephania tetrandra S.Moore
摘要
Abstract
Objective:To observe the effect of inflammatory factors from decoction of Stephania tetrandra S.Moore and its different split components that lipopolysaccharide (LPS)was induced by RAW264.7 cells.Methods:Use MTT method to determine the cytotoxicity of different parts from the decoction and its split components of RAW264.7 cells.Use Gries'reagent ( Griess method) to determine the NO content of the inflammatory reaction cell and use the method of enzyme-linked immunosorbent (ELISA) to detect tumor necrosis factor alpha (TNF-α) and interleukin 6 (IL-6) content in the supernatant fluid.Results: The decoction and its alkaloids components could inhibit LPS induced by RAW264.7 cells releasing inflammatory factor NO ( P<0.01) , the TNF-α( P<0.01) , IL-6 ( P<0.01) and other components had no obvious effects.Conclusion:The concentrations of decoction and its different parts are less than 312.50mg/L for RAW264.7 cells and have no significant toxicity.The decoction and its alkaloids split components may inhibit the re-lease of cytokines NO, TNF-α, IL-6 and exert its anti-inflammatory effects.关键词
防己/炎症/小鼠巨噬细胞/酶联免疫吸附试验/细胞因子Key words
Stephania tetrandra S.Moore/Inflammation/Macrophages in mice/ELISA/Cytokines分类
医药卫生引用本文复制引用
郭辰,王蒙,李静,魏晴,王秋红,杨炳友,匡海学..防己水煎液及其不同拆分部位对脂多糖诱导下 RAW264．7细胞炎症因子的影响[J].中医药学报,2015,(4):33-36,4.基金项目
国家重点基础研究发展计划（973计划） （）
