重庆医学Issue(23)：3169-3171,3175,4.DOI:10.3969/j.issn.1671-8348.2015.23.001
转基因番茄可食防龋疫苗免疫大鼠的实验研究∗
The study of oral immunization with the transgenic tomato anti-caries vaccine in SD rats
摘要
Abstract
Objective SD rats were immuned with the transgenic tomatoes which carried fused gene of a region of PAc Strep-tococcus mutants and cholera toxin B subunit.The immunogenicity was tested to explore secure and economic edible vaccines a-gainst dental caries.Methods A total of 18 eighteen-day-old female SD rats were subdivided randomly into three groups:the exper-imental group which were fed with transgenic tomato juice containing chimaera protein PAcP/CTB;the positive control group which were treated with deactivated S.mutans;the negative control group which were not treated with transgenic tomato juice.Rats were immuned once per week for four weeks.Blood and saliva were collected at one day before the first immunity and one week after each immunization.IgG of blood serum and SIgA of saliva were detected using the enzyme-linked immunosorbent assay (indirect ELISA) testing.On day 70,rats were terminated.The maxillary and mandibular bones were subsequently taken out to count dental caries′scores.Results Post immunization,the experimental group and the positive control group had statistical significant levels of speci-ficity IgG in serum and SIgA in saliva compared to the negative control group (P <0.05).There was a significance difference be-tween the experimental group and the negative control group except in Dx levels of caries loss (P <0.05).Conclusion The targeted protein expressed on the transgenic tomatoes is immunogenic,which can effectively induce mucous membrane immune response and the systematical immunoreaction to suppress the occurrence of the dental caries.关键词
番茄/植物,基因修饰/免疫疗法/龋齿Key words
lycopersicon esculentum/plants,genetically modified/immunotherapy/dental caries分类
医药卫生引用本文复制引用
顾瑜,刘建国,白国辉,吴家媛,管晓燕,陈筑,白朋元,赵靖..转基因番茄可食防龋疫苗免疫大鼠的实验研究∗[J].重庆医学,2015,(23):3169-3171,3175,4.基金项目
国家自然科学基金资助项目(30160086) （30160086）
贵州省第6批科技创新团队资助项目[黔科合人才团队(2013)4026号] （2013）
贵州省高等学校重点学科建设项目(SZXK-201207-04)。 （SZXK-201207-04）
