重庆医学Issue(24)：3328-3330,3.DOI:10.3969/j.issn.1671-8348.2015.24.006
高压氧对兔颈内动脉和基底动脉内皮细胞凋亡影响的实验研究∗
Apoptotic effect of HBO exposure on cerebral vascular endothelial cells in rabbits
摘要
Abstract
Objective To investigate the effects of hyperbaric Oxygen (HBO)exposure on Caspase-3 and Bcl-2 mRNA ex-pressions in both internal carotid artery (ICA)and basal artery (BA)in rabbits.Methods Twenty-four healthy adult New Zealand rabbits were randomly divided into 2 groups:HBO group and the control group,with each group consisted of 12 animals.The rab-bits in the HBO group were exposed to HBO at 2.2 ATA for 60 minutes each day for 3 successive days.The rabbits in the control group were normally fed without any treatment.Real-time PCR was used to detect Caspase-3 and Bcl-2 mRNA expressions in both ICA and BA in 2 groups.Results HBO significantly decreased the Caspase-3 mRNA expression [(0.038 ±0.006 )vs .(1.000 ± 0.225)]and increased the Bcl-2 mRNA expression [(1.877 ±0.1 69)vs .(1.000 ±0.364)].In ICA,HBO similarly decreased the Caspase-3 mRNA expression [(0.41 9±0.091)vs .(1.000 ±0.1 75)]and increased the Bcl-2 mRNA expression [(1.269 ±0.270) vs .(1.000±0.1 1 7)]in BA.All the differences mentioned above were of statistical significance (P <0.01).Conclusion HBO ex-erts an inhibition effect on apoptosis in cerebral vascular endothelial cells.The mechanism may be related to inhibiting the expres-sion of Caspase-3 mRNA and promoting the expression of Bcl-2 mRNA.关键词
高压氧/颈内动脉/基底动脉/细胞凋亡/Caspase-3/Bcl-2/实时荧光定量 PCRKey words
hyperbaric Oxygen/internal carotid artery/basal artery/cell apoptosis/Caspase-3/Bcl-2/Real-time F.Q.PCR分类
医药卫生引用本文复制引用
范祯祯,刘志艳,蔡宏斌,张翠杰,张旭东,张兰芳,梁成,葛朝明..高压氧对兔颈内动脉和基底动脉内皮细胞凋亡影响的实验研究∗[J].重庆医学,2015,(24):3328-3330,3.基金项目
甘肃省自然科学研究基金计划(1107RJZA082)。 （1107RJZA082）
