吉林医药学院学报Issue(5)：324-327,4.
芝麻素对人结肠癌SW480细胞株的影响
The effect of sesamin on human’s colon cancer SW480 cells
摘要
Abstract
Objective To investigate the effect of Sesamin on human’s colon cancer SW480 cells and its mechanism. Methods MTT colorimetry was employed to determine the median inhibitory concentration ( IC50 ) and observe the influence of Sesamin on SW480 cells’ proliferation. SW480’s morphological changes were observed under the micro-scope. Annexin-FITC/PI double staining was used to detect the cells’ apoptosis rate. The expression of caspase-3 and caspase-8 were observed by the immunohistochemical staining. Results Seatrain inhibited the proliferation of SW480;SW480'volume shrinks and becomes round,while the phenomenon of apoptosis was clear. Compared with the control group,the Sesamin group’s early apoptosis rate is statistically significant (P <0. 01) and the expression of caspase-3 and caspase-8 was more significant ( P <0 . 01 ) . Conclusion Sesamin could inhibit the proliferation of SW480 in a dose-depend manner and its mechanism may be concerned in the activation of caspase family members.关键词
芝麻素/结肠癌/SW480细胞Key words
sesamin/colon cancer/SW480 cell分类
医药卫生引用本文复制引用
孙方博,白金权,王瑶,赵斌,佟瑶瑶,张晓霞..芝麻素对人结肠癌SW480细胞株的影响[J].吉林医药学院学报,2015,(5):324-327,4.基金项目
吉林医药学院大学生创新创业训练计划项目资助 （）
