动物营养学报Issue(8)：2368-2377,10.DOI:10.3969/j.issn.1006-267x.2015.08.008
高脂饲粮对日本鹌鹑（Cotur nix japonica）盲肠微生物群落结构的影响
Effects of High Lipid Diet on Cecal Microbial Community Structure of Japanese Quail (Cotur nix japonica)
刘莎莎 1张宏福 2郑明德 1计成3
作者信息
- 1. 中国农业科学院北京畜牧兽医研究所，动物营养国家重点实验室，北京 100193
- 2. 中国农业大学动物科学技术学院，北京 100193
- 3. 加拿大英属哥伦比亚大学，禽类研究中心，温哥华 V6T1Z4
- 折叠
摘要
Abstract
The object of this study was to investigate the effects of high lipid diet on cecal microbial community structure and composition and its lipid metabolism of Japanese quail ( Cotur nix japonica) . A total of 160 one-day-old Japanese quail fed a semi-synthetic diet for the first 6 weeks. At 6 weeks of age, the birds were ran-domly divided into 2 groups with 8 replicates per group and 10 birds per replicate, fed either a regular synthetic diet or a synthetic diet with added cholesterol for another 6 weeks. After the feeding trial, 8 birds from each group were euthanized and collected blood for testing the lipid metabolism indices, cecal contents for investiga-ting the microbial community by using pyrosequencing.The results showed as follows:1) The content of total cholesterol ( TC) , low density lipoprotein ( LDL) and LDL/high density lipoprotein ( HDL) of Japanese quail were significantly increased in high lipid diet group ( P<0.05) . 2) High lipid diet significantly decreased cecal microbial diversity ( P<0.05); in high lipid diet group, the abundance of family Lactobacillales, Streptococ-caceae and Clostridiales, others was significantly decreased ( P<0.05) , while the abundance of family Erysip-elotrichales, Erysipelotrichaceae was significantly increased ( P<0.05) . 3) The abundance of cc_115 ( Erysip-elotrichaceae) and unclassified bacteria belong to Lachnospiraceae were positively correlated with contents of TC, LDL and LDL/HDL ( P<0.05) , besides Eubacterium ( Erysipelotrichaceae) was only positively correla-ted with LDL/HDL (P<0.05), while the abundance of Ruminococcus (Ruminococcaceae) was negatively correlated with contents of TC, LDL and LDL/HDL ( P<0.05) , and the abundance of Ruminococcus( Lach-nospiraceae) was positively correlated with contents of HDL ( P<0.05) . In conclusion, high lipid diet highly affects the cecal microbial community structure and composition and its lipid metabolism of Japanese quail.关键词
高脂饲粮/肠道微生物群落/脂肪代谢Key words
high lipid diet/cecal microbial community/lipid metabolism分类
农业科技引用本文复制引用
刘莎莎,张宏福,郑明德,计成..高脂饲粮对日本鹌鹑（Cotur nix japonica）盲肠微生物群落结构的影响[J].动物营养学报,2015,(8):2368-2377,10.
