中国耳鼻咽喉头颈外科Issue(8)：426-430,5.DOI:10.16066/j.1672-7002.2015.08.007
组胺对呼吸道上皮紧密连接表达的影响
Role of histamine on the expression of tight junctions gene in airway epithelia
摘要
Abstract
OBJECTIVE To investigate and elucidate the role of histamine on the regulation of tight junctions(TJs) gene expression of the airway epithelial cells. METHODS We cultured primary human bronchial epithelial cells from healthy and asthmatic individuals in the presence of histamine for 96 hours in air-liquid interface (ALI) culture system. The TJs expression on the level of mRNA and protein was determined by RT-qPCR and immunofluorescence (confocal microscopy), respectively. RESULTS A RT-qPCR analysis revealed that the mRNA expression of several TJs were up- or down-regulated by histamine. However, examination by confocal microscope revealed no detectable alterations in the morphology of the tight junctions after stimulating with histamine. CONCLUSION Our findings that histamine could regulate the mRNA expression of TJs of bronchial epithelial cells. That might contribute to the pathogenesis of chronic airway inflammatory diseases.关键词
组胺/呼吸道黏膜/紧密连接部Key words
Histamine/Respiratory Mucosa/Tight Junctions引用本文复制引用
段甦,矫健,张罗..组胺对呼吸道上皮紧密连接表达的影响[J].中国耳鼻咽喉头颈外科,2015,(8):426-430,5.基金项目
国家自然科学基金重点国际合作研究项目（81420108009）、科技部“十二五”国家科技支撑计划课题（2014BAI07B04）、教育部创新团队发展计划（IRT13082）、北京市自然科学基金（7131006）和北京市医管局重点医学专业发展计划（ZYLX201310）联合资助 （）
