福州大学学报（自然科学版）Issue(4)：554-559,6.DOI:10.7631/issn.1000-2243.2015.04.0554
悬浮固化液相微萃取－高效液相色谱法分析生长素
Analysis of auxins by solidification of floating organic drop combined with high-performance liquid chromatography
摘要
Abstract
Determination of two anxins ( indole propionic acid and indole butyric acid) by solidifica-tion of floated organic drop microextraction with high -performance liquid chromatography-fluores-cence detection ( SFODME-HPLC-FLD) was established.The experimental conditions affecting the extraction process were optimized.The optimal conditions of SFODME were as follows:50μL dode-canol as extraction solvent, 20.0 mL sample solution with pH 3.0 and 0.25 g · mL-1 NaCl.The above solution was extracted for 10 min at temperature of 26 ℃ and centrifugation speed of 1 800 r· min-1 .Results showed that this SFODME approach had high sensitivity and good reproducibility, with relative standard deviations lower than 8.66%.Its linear range was 0.02~60 ng· mL-1 , with the limit of detection of 0.01 ng · mL-1 and the recoveries larger than 84.3%.This method was rapid, stable and environmentally benign, and it can be applied to real sample analysis.关键词
悬浮固化液相微萃取/高效液相色谱/液相微萃取/生长素Key words
solidified floating organic drop microextraction/high -performance liquid chromatogra-phy/liquid phase microextraction/auxin分类
化学化工引用本文复制引用
卢巧梅,张文敏,黄川辉,卢明华,励建荣,张兰..悬浮固化液相微萃取－高效液相色谱法分析生长素[J].福州大学学报（自然科学版）,2015,(4):554-559,6.基金项目
“十二五”国家科技支撑计划项目（2012BAD29B06）；福建省自然科学基金资助项目（2010J05021）；福州大学科技启动专项 （）
