广东牙病防治Issue(9)：457-461,5.
磷脂酰胆碱在伴放线放线杆菌粘附和侵袭过程中的作用∗
Role of phosphorylcholine in Aggregatibacter actinomycetemcomitans adhesion and invasion
摘要
Abstract
Objective To explore the role of phosphorylcholine ( PC) during Aggregatibacter actinomycetemcomitans ( Aa) adhesion and invasion of human umbilical vein endothelial cells ( HUVEC) . Methods Non-attached plaque in periodontal pockets of 7 patients with aggressive periodontitis was collected and then inoculated on the selective medium TSBV plate. The phosphorylcholine positive expression Aa clinical strains were isolated through colony morphology, cata-lase test, Gram stain experiment, 16S rDNA and bacterial protein expression. Then, adhesion and invasion tests were taken to explore the role of phosphorylcholine during Aa pathogenisis process. Results After selective culture and spe-cific identification, one phosphorylcholine positive expression Aa clinical strain was found by Western blot. After pretrea-ted with anti-PC monoclonal antibody TEPC-15, the effect of Aa adhesion to and invasion of HUVEC was obviously de-clined ( 31. 87 ± 4. 22)% and ( 24. 63 ± 3. 55)% respectively. Conclusion PC helps PC positive Aa adhesion to and in-vasion of HUVEC.关键词
伴放线放线杆菌/磷脂酰胆碱/粘附与侵袭Key words
Aggregatibacter actinomycetemcomitans/Phosphorylcholine/Adhesion and invasion分类
医药卫生引用本文复制引用
王勤,张雄,钟德钰,轩东英,谢成婕,刘伟珍,章锦才..磷脂酰胆碱在伴放线放线杆菌粘附和侵袭过程中的作用∗[J].广东牙病防治,2015,(9):457-461,5.基金项目
国家自然科学基金项目(81371151) （81371151）
广东省医学科学技术研究基金项目(A2014112) （A2014112）
