临床与病理杂志Issue(8)：1511-1518,8.DOI:10.3978/j.issn.2095-6959.2015.08.017
高压氧治疗对2型糖尿病大鼠肝脏超微结构及细胞凋亡的影响
Effects of hyperbaric oxygen treatment on the tissue apoptosis and ultrastructure of liver cells in rats with type 2 diabetes
摘要
Abstract
Objective:hTe major objective was to explore the protection machanism of hyperbaric oxygen treatment on liver cells of GK (Goto Kakizaki) rats with type 2 diabetes mellitus.Methods: GK rats (n=24) were divided randomly <br> and evenly into model group (received gastric irrigation with puriifed water 5 mL/kg/d once per day), metformin hydrochloride group (MH,n=8, received gastric irrigation with metformin hydrochloride 250 mg/kg/d once daily), and hyperbaric oxygen group (HBO,n=8, inhaled pure oxygen under a constant pressure 0.15 Mpa for 30 min per day, and received gastric irrigation with puriifed water 5 mL/kg/d once per day), while healthy Wistar rats were used as normal control group (N,n=8, received gastric irrigation with puriifed water 5 mL/kg/d once per day). After 3 weeks of treatment, rats were fasted for 12 hours, measured body weight and fasting blood glucose. And then the rats were anesthetized with isolfurane, and immediately separated the liver, liver tissue were taken electron microscopic examination and biopsy paraffn. Some paraffn sections were stained using PAS and Masson, some samples were stained using the immunohistochemistry approach with Caspase-3 anti-bodies, the apoptosis of liver cells was examined using the TUNEL technique.Results: hTese resultant data showed that the pathological changes of the HBO group was less than those of the model group with respect to hepatic steatosis, fibrosis, glycogen increases, increased apoptosis and mitochondrial swelling, degeneration. The index of cell apoptosis and Caspase-3 expression in the HBO group showed signiifcant difference (P<0.05) with that in the model control, and no signiifcant difference (P>0.05) compared with that in the normal control and metformin hydrochloride group.Conclusion: hTis implies that the HBO treatment might protect liver tissues, improve the structure of the liver by easing increased apoptosis induced by hyperglycemia in diabetic rat liver.关键词
高压氧/细胞凋亡/超微结构/2型糖尿病/大鼠/肝脏Key words
hyperbaric oxygen treatment/apoptosis/ultrastructure/type 2 diabetes mellitus/Goto Kakizaki rats/liver引用本文复制引用
聂文洁,曹秀琴,邵贵强..高压氧治疗对2型糖尿病大鼠肝脏超微结构及细胞凋亡的影响[J].临床与病理杂志,2015,(8):1511-1518,8.基金项目
上海市闵行区卫计委科研课题(2012MW18)，上海市闵行区科委自然科学研究课题(2015MHZ063)。@@@@This work was supported by National Health and Family Planning Commission of Minhang District of Shanghai (2012MW18) and Science and Technology Commission of Minhang District of Shanghai (2015MHZ063), P. R. China （2012MW18）
