郑州大学学报（医学版）Issue(5)：627-630,4.DOI:10.13705/j.issn.1671-6825.2015.05.009
氟暴露儿童 ERα基因甲基化水平与氟斑牙及血清钙水平的关系
Relationship between dental fluorosis, serum calcium level, and ERαgene methylation in children exposed to fluoride
摘要
Abstract
Aim:To explore the relationship between estrogen receptor α( ERα) gene methylation and children dental fluorosis,serum calcium level .Methods:A total of 174 primary school students aged 8~12 years, born and raised up in fluoride exposure areas of Henan Province were selected by cluster sampling , and were divided into normal group(n=85), mild group(n=53), as well as moderate and severe group (n=36) according to dental fluorosis degree .Dental fluorosis was identified with Dean method .Urine fluoride and serum calcium levels were determined using fluoride ion selective elec -trode and flame atomic absorption spectrometry methods , respectively .The methylation level of ERαgene promoter was de-tected by real-time PCR procedure .Results:The percentage of ERαgene methylation in the boys of moderate and severe group[(2.61 ±0.95)%] was significantly higher than those in normal group [(1.89 ±0.74)%] and mild group [(1.99 ±0.89)%](P<0.05).No significant difference in ERαgene methylation percentage was observed among the girls from different groups(F=0.585,P=0.559).Negative correlation between methylation level in ERαgene and serum calcium level of children was observed (r=-0.172,P=0.024).Conclusion:ERαgene methylation may be related with the degree of children dental fluorosis and this may have gender specificity .There is a negative correlation between the methylation level of ERαgene and serum calcium level .关键词
氟斑牙/雌激素受体α/DNA甲基化/血清钙Key words
dental fluorosis/estrogen receptor α/DNA methylation/serum calcium分类
医药卫生引用本文复制引用
王科妍,崔蕊蕊,丁中,崔留欣,赵明旭,程学敏,巴月..氟暴露儿童 ERα基因甲基化水平与氟斑牙及血清钙水平的关系[J].郑州大学学报（医学版）,2015,(5):627-630,4.基金项目
国家自然科学基金资助项目81072247；河南省教育厅科学技术研究重点项目13 A330653 （）
