华西口腔医学杂志Issue(4)：347-351,5.DOI:10.7518/hxkq.2015.04.005
细胞间隙连接蛋白43基因在斑马鱼牙齿早期发育中的表达
Expression of connexin 43 gene during early dental development in zebra fish
摘要
Abstract
Objective This study aims to investigate the expression of connexin 43 (cx43) gene during early development in zebra fish and provide a foundation for further research of cx43 gene in tooth development. Methods Total RNA was extracted within 72 h after fertilization of zebra fish embryos and then reversed transcribed to generate the cDNA library. The specific fragments of the cx43 gene were then cloned and connected to the PGEMT vector. After confirming the constructed plasmid, the corresponding RNA polymerase was chosen, and the digoxin-labeled anti-sense mRNA probe of cx43 was syn-thesized in vitro. The cx43 gene expression of zebra fish indifferent stages was carried out by in situ hybridization. The rela-tionship of the cx43 gene expression and anatomy of the pharyngeal teeth were compared by alizarin red staining. Results The mRNA antisense probe of cx43 was acquired. The positive signal of sepia was observed in the different stages of zebra fish pharyngeal teeth after fertilization. After fertilization for 9 days, the expression site of cx43 in situ hybridization was over-lapped in accordance with the anatomical site of the pharyngeal teeth. Conclusion cx43 gene participates in tooth develop-ment and mineralization process and plays a crucial role in later mineralization.关键词
细胞间隙连接蛋白43基因/原位杂交/斑马鱼Key words
connexin 43 gene/in situ hybridization/zebra fish分类
生物科学引用本文复制引用
徐智云,刘鑫,黄兴,杨德琴..细胞间隙连接蛋白43基因在斑马鱼牙齿早期发育中的表达[J].华西口腔医学杂志,2015,(4):347-351,5.基金项目
国家自然科学基金资助项目（31371473）；重庆市医学重点学科建设基金资助项目 （）
