华西口腔医学杂志Issue(4)：383-387,5.DOI:10.7518/hxkq.2015.04.012
慢性间歇低氧对大鼠牙周炎模型血清及牙龈组织中白细胞介素-18和低氧诱导因子-1α的影响
Effects of interleukin-18 and hypoxia-inducible factor-1αin serum and gingival tissues of rat model with periodontitis ;exposed to chronic intermittent hypoxia
摘要
Abstract
Objective This study evaluates the expression of interleukin-18 (IL-18) and hypoxia-inducible factor (HIF)-1αin rat periodontitis model exposed to normoxia and chronic intermittent hypoxia (CIH) environments. The possible correlation between periodontitis and obstructive sleep apnea-hypopnea syndrome (OSAHS) was also investigated. Methods Thirty-two Sprague–Dawley (SD) rats were randomly assigned into four groups: normoxia control, normoxia periodontitis, hypoxia control, and hypoxia periodontitis groups. The periodontitis models were established by ligating the bilateral maxillary second molars and employing high-carbohydrate diets. Rats in hypoxia control and hypoxia periodontitis groups were exposed to CIH treatment mimicking a moderately severe OSAHS condition. All animals were sacrificed after eight weeks, and the clinical periodontal indexes were detected. The levels of IL-18 and HIF-1αin serum and gingival tissues were determined using enzyme-linked immunosorbent assay (ELISA). The correlation between attachment loss (AL) and the levels of IL-18 and HIF-1αin hypoxia periodontitis group was evaluated. Results The levels of IL-18 and HIF-1αin hypoxia periodontitis group were significantly higher than that in normoxia periodontitis and hypoxia control groups (P<0.05). Furthermore, the levels of IL-18 and HIF-1αin serum (r=0.792, r=0.753, P<0.05) and gingival tissues (r=0.817, r=0.779, P<0.05) were posi-tively correlated with AL. Conclusion CIH could aggravate the destruction of periodontal tissues, which is correlated with IL-18 and HIF-1αlevels.关键词
慢性间歇低氧/阻塞性睡眠呼吸暂停低通气综合征/牙周炎/白细胞介素-18/低氧诱导因子-1αKey words
chronic intermittent hypoxia/obstructive sleep apnea-hypopnea syndrome/periodontitis/interleukin-18/hypoxia-inducible factor-1α分类
医药卫生引用本文复制引用
王斌,王小琴..慢性间歇低氧对大鼠牙周炎模型血清及牙龈组织中白细胞介素-18和低氧诱导因子-1α的影响[J].华西口腔医学杂志,2015,(4):383-387,5.基金项目
山西省自然科学基金资助项目 （）
