吉林中医药Issue(8)：837-840,4.DOI:10.13463/j.cnki.jlzyy.2015.08.025
通莲汤对裸小鼠胃癌移植瘤生长及缺氧诱导因子-1α的影响
Tonglian Decoction on tumor growth and HIF-1α expression of tumor tissue from nude mice modeled with gastric cancer cells
摘要
Abstract
Objective The purpose of the research is to observe inhibition of Tonglian Decoction (TD ) on nude mice mod-eled with gastric cancer cells MGC-803 and on hypoxia inducible factor-1α(HIF-1α) expression in tumor tissue ,to provide experimental basis for clinical application of TD .Methods MGC-803 cells are inoculated subcutaneously in right forelimbs of all mice .Mice in model group are treated with saline solution 10 mL/kg orally .Mice in positive control group are treated with Xiaoaiping tablet ,dosage of 10 mL/kg ,1 .8 g/kg (crude drug ) of liquid .Mice in TD groups are subdivided as high (1 . 5 g/L ) ,medium (0 .75 g/L ) and low (0 .375 g/L ) dose group of drug liquid ,with dosage of 10 mL/kg .All mice take ad-ministration for 3 weeks and are observed for another 3 weeks continuously .Tumor volumes are measured for 6 weeks every-day from the beginning of MGC803 cells transplanted .All mice are killed for isolation of tumor tissue ,inhibition ratio of tu-mor-weight measured .Finally ,HIF-1α expression is examined with immunohistochemistry .Results Tumor weight in TD high-dose group is (1 .51 ± 0 .49 ) g ,and the inhibition ratio is (61 .04 ± 4 .79 )% ,which is significantly different from that in the model group ( P<0 .05 );Positive rate of HIF-1αexpression also decreased significantly ,showing statistical difference from the model group (χ2=4 .286 ,P=0 .038<0 .05 ) .Conclusion TD inhibits tumor growth on nude mice modeled with MGC803 ,through downregulation of HIF-1αprotein expression .关键词
通莲汤/胃癌/MGC803细胞/荷瘤裸鼠/缺氧诱导因子-1αKey words
Tonglian Decoction/gastric cancer/MGC803 cells/nude mice transplanted with tumor/HIF-1α分类
医药卫生引用本文复制引用
张艳丽,贾永森,秦丽娟,孙瑞军..通莲汤对裸小鼠胃癌移植瘤生长及缺氧诱导因子-1α的影响[J].吉林中医药,2015,(8):837-840,4.基金项目
河北省自然科学基金（H2013209053）。 （）
