检验医学与临床Issue(16):2342-2343,2.DOI:10.3969/j.issn.1672-9455.2015.16.017
全自动酶联免疫分析仪检测乙肝核心抗体的加样质量控制
Quality control of adding sample on HBcAb by automatic enzyme immunoassay instrument
摘要
Abstract
Objective To select the ideal examination program of the TECAN automatic enzyme immunoassay instrument for detecting HBcAb .Methods The different adding modes and adding sequences were set on the same sample for obtaining the corresponding data .Results In detecting HBcAb by the TECAN automatic enzyme immu‐noassay instrument ,adding sample needle had the entrainment phenomenon ,which could cause the sample contamina‐tion ,thus led to false positive results or contaminated the negative control to make the CO value decrease and cause the false negative result .The improvement of adding sample program reduced the carrying probability ,avoided the negative control pollution and increased the detection quality .Conclusion Adopting the single adding sample mode , and first adding negative and positive control and then sample are the ideal detection program .关键词
乙肝核心抗体/全自动酶联免疫分析仪/质量控制Key words
HBcAb/automatic enzyme immunoassay instrument/quality control引用本文复制引用
张龙,银广悦,武彩虹,王辉,张朝云..全自动酶联免疫分析仪检测乙肝核心抗体的加样质量控制[J].检验医学与临床,2015,(16):2342-2343,2.基金项目
河北省廊坊市科技局项目(2013013020C)。 ()