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全自动酶联免疫分析仪检测乙肝核心抗体的加样质量控制

张龙 银广悦 武彩虹 王辉 张朝云

检验医学与临床Issue(16):2342-2343,2.
检验医学与临床Issue(16):2342-2343,2.DOI:10.3969/j.issn.1672-9455.2015.16.017

全自动酶联免疫分析仪检测乙肝核心抗体的加样质量控制

Quality control of adding sample on HBcAb by automatic enzyme immunoassay instrument

张龙 1银广悦 1武彩虹 1王辉 2张朝云3

作者信息

  • 1. 中国石油中心医院 检验科,河北廊坊 065000
  • 2. 中国石油中心医院 急诊科,河北廊坊 065000
  • 3. 中国石油中心医院 内分泌科,河北廊坊 065000
  • 折叠

摘要

Abstract

Objective To select the ideal examination program of the TECAN automatic enzyme immunoassay instrument for detecting HBcAb .Methods The different adding modes and adding sequences were set on the same sample for obtaining the corresponding data .Results In detecting HBcAb by the TECAN automatic enzyme immu‐noassay instrument ,adding sample needle had the entrainment phenomenon ,which could cause the sample contamina‐tion ,thus led to false positive results or contaminated the negative control to make the CO value decrease and cause the false negative result .The improvement of adding sample program reduced the carrying probability ,avoided the negative control pollution and increased the detection quality .Conclusion Adopting the single adding sample mode , and first adding negative and positive control and then sample are the ideal detection program .

关键词

乙肝核心抗体/全自动酶联免疫分析仪/质量控制

Key words

HBcAb/automatic enzyme immunoassay instrument/quality control

引用本文复制引用

张龙,银广悦,武彩虹,王辉,张朝云..全自动酶联免疫分析仪检测乙肝核心抗体的加样质量控制[J].检验医学与临床,2015,(16):2342-2343,2.

基金项目

河北省廊坊市科技局项目(2013013020C)。 ()

检验医学与临床

OACSTPCD

1672-9455

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