检验医学与临床Issue(16)：2346-2348,3.DOI:10.3969/j.issn.1672-9455.2015.16.019
血小板裂解液差异蛋白在原发免疫性血小板减少症诊断中的临床应用研究
Study of clinical study of platelet lysate differential protein in diagnosis of primary immune thrombocytopenia
摘要
Abstract
Objective To establish a quick and simple diagnostic laboratory method with high sensitivity and good specificity for the diagnosis of primary immune thrombocytopenia ITP by studying platelet lysate differential protein in the patients with ITP for understanding its relationship with the onset of ITP .Methods The platelet ly‐sate was detected in 64 cases of ITP and 42 cases of healthy people by surface enhanced laser desorption/ionization‐time of flight‐mass spectrometry(SELDI‐TOF‐MS) technology for obtaining the platelet protein mass spectrum .The differential protein was screened and combined with the artificial neural network(ANN) for establishing the diagnos‐tic model of ITP .Results The protein with the mass electron ratio(m/z) of 3 549 .17 and 7 678 .09 was highly ex‐pressed in the ITP group and which of m/z 5 328 .29 and 7 894 .32 was lowly expressed .The sensitivity and specifici‐ty of the diagnostic model were 93 .3% and 82 .6% respectively .Conclusion The established ANN model on the ba‐sis of platelet protein mass spectrum may have some clinical value for the diagnosis of ITP .关键词
原发免疫性血小板减少症/蛋白质组学/表面增强激光解吸电离飞行时间质谱Key words
primary immune thrombocytopenia/proteomics/SELDI-TOF-MS引用本文复制引用
吴鹏强,汤计瑞,韩丽英..血小板裂解液差异蛋白在原发免疫性血小板减少症诊断中的临床应用研究[J].检验医学与临床,2015,(16):2346-2348,3.基金项目
泸州医学院青年基金项目（12065）。 （）
