农业资源与环境学报Issue(4)：354-362,9.DOI:10.13254/j.jare.2014.0353
施氮对间作条件下玉米、马铃薯根际微生物群落功能多样性的影响
Effects of Nitrogen Application Rates on Rhizosphere Microbial Community Functional Diversity in Maize and Potato Intercropping
摘要
Abstract
Field trials were carried out to investigate the effects of different nitrogen application rates N0(0 kg·hm-2), N1(125 kg·hm-2), N2 (250 kg·hm-2)and N3(375 kg·hm-2)on the rhizosphere microbial population and metabolic function diversity of maize and potato under intercropping using plate culture method and BIOLOG technique. The results indicated that nitrogen(N1, N2 and N3)application in-creased the amounts of bacteria, actinomyces and total microbes, but decreased the quantities of fungi significantly in rhizosphere soil of maize and potato in intercropping, and the highest increment was with N2 treatment. In comparison with N0, nitrogen fertilizer application could increase significantly the diversities of soil microbial community, the utilization rate of carbon source, richness of soil microbial com-munity. And the A W CD value, Shannon-Wiener index(H), Simpson index(D), Evenness index(E)and Richness index(S)in rhizosphere soil of maize under intercropping were the highest at N3 treatment, while that of potato were the highest at N2 treatment, but the effects of dif-ferent N application rates on the ability of rhizospheric microbes in utilizing six types of carbon sources were different. Principal component analysis (PCA)and cluster analysis showed that there were differences in carbon substrate utilization patterns and metabolic characteristics of the soil microbes in maize and potato intercropping with different N application rates. It suggested that applying N could regulate the rhizo-sphere soil microbial communities and promote the functional diversity of crop intercropping.关键词
玉米/马铃薯间作/施氮量/土壤微生物群落/功能多样性Key words
maize/potato intercropping/nitrogen application rate/soil microbial community/functional diversity分类
农业科技引用本文复制引用
覃潇敏,郑毅,汤利,龙光强..施氮对间作条件下玉米、马铃薯根际微生物群落功能多样性的影响[J].农业资源与环境学报,2015,(4):354-362,9.基金项目
国家自然科学基金 （）
