山东医药Issue(30)：5-7,3.DOI:10.3969/j.issn.1002-266X.2015.30.002
褐藻多糖对乳腺癌细胞活性的影响及机制探讨
Effects of Fucoidan on cell viability of breast cancer and its mechanism
摘要
Abstract
Objective To observe the effects of Fucoidan on cell viability of breast cancer cell lines MCF -7 and MDA-MB-231 and to investigate its possible mechanism .Methods The breast cancer cell lines MCF-7 and MDA-MB-231 in the logarithmic phase ( observation group A and B ) were respectively treated with 2 mL Fucoidan ( 100 ug/mL and 200 ug/mL) .The control group A and B were treated with the same volume of DMEM culture medium .The cell proliferation inhi-bition rate was detected by Trypan blue staining , cell cycle and apoptosis cells were analyzed by flow cytometry , and the expression of Caspase-8, phosphorylation Erk ( p-Erk) and total Erk was detected by Western blotting .Results With the increased Fucoidan concentration and the prolonged time , the cell proliferation inhibition rates of the observation groups A and B were increased (all P<0.05), the cell proportion in the G0/G1 phase of the observation groups A and group B was increased, the cell proportion in the S phase and G2 was reduced, the apoptosis rate was increased, and the changes in the high concentration Fucoidan group were more significant (all P<0.05).The expression of Caspase-8 was increased and the Erk expression was decreased in the observation groups A and B , and the changes in the high concentration Fucoidan group were more significant (all P<0.05).Conclusion Fucoidan could inhibit the proliferation of MCF-7 and MDA-MB-231 cell lines, arrest the breast cells in G0/G1 phase, and also promote the apoptosis.This mechanism may be achieved by activating the expression of Caspase-8 and down-regulating the p-Erk expression .关键词
褐藻多糖/乳腺癌/细胞周期/细胞凋亡/半胱氨酸天冬氨酸蛋白酶8/细胞外调节蛋白激酶Key words
Fucoidan, breast carcinoma/cell cycle/apoptosis/caspase/extracellular signal-regulated kinases分类
医药卫生引用本文复制引用
郝剑,吴雄志..褐藻多糖对乳腺癌细胞活性的影响及机制探讨[J].山东医药,2015,(30):5-7,3.基金项目
国家自然科学基金资助项目（81173376）；新世纪优秀人才基金资助项目（NCET-11-1068）。 （）
