山东医药Issue(30)：8-10,3.DOI:10.3969/j.issn.1002-266X.2015.30.003
猴头多糖对小鼠皮下移植肉瘤的抑制作用及机制探讨
Inhibitory effect of Hericium erinaceus polysaccharide on mice subcutaneous transplanted sarcoma and its mechanism
摘要
Abstract
Objective To observe the inhibitory effect of Hericium erinaceus polysaccharide on the mice subcutaneous transplanted sarcoma , and to explore its mechanism from apoptosis perspective .Methods S180 sarcoma cells were subcu-taneously injected into 50 mice to produce the models of subcutaneous tumor transplantation .After successful modeling , the tumor bearing mice were randomly divided into five groups , 10 in each group: the low-dose group , medium-dose group , high-dose group, model group and cyclophosphamide group .The low-dose, medium-dose and high-dose groups were given hedgehog polysaccharide 25, 50 and 100 mg/kg, orally, once a day, and for 10 days.The model group was given normal saline 0.2 mL/day orally and for 10 days.Cyclophosphamide was administered with 40mg/kg by intraperitoneal injection once a day for 5 days.All the mice were killed after 10 days of treatment .We calculated the inhibition rate of each group . The apoptosis in each group was detected by TUNEL assay .Caspase-3 protein was detected by immunohistochemical meth-od.Results The tumor inhibition rates in the low-dose group, medium-dose group, high-dose group and cyclophospha-mide group were 36.36%, 57.57%, 43.93%and 74.24%.The tumor inhibition rate in the cyclophosphamide group was higher than those in the other groups , and in the medium-dose group , the tumor inhibition rate was higher than those of the low-dose group and high-dose group (all P <0.05).The number of apoptotic cells and Caspase-3 protein expression were as follows:cyclophosphamide group >medium-dose group >high-dose group >low-dose group >model group ( all P<0.05).Conclusion Hericium erinaceus polysaccharide can inhibit the growth of xenograft sarcoma , and the dose of 50 mg/kg dose is the best .This mechanism may be related to its up-regulation of Caspase-3 protein expression and thereby promoting tumor cell apoptosis .关键词
猴头多糖/肉瘤/细胞凋亡/凋亡相关蛋白Key words
Hericium erinaceus polysaccharide/sarcoma/apoptosis/apoptosis-related protein分类
医药卫生引用本文复制引用
姜颖,于英君,刘国良,齐彦,李红影,孙力..猴头多糖对小鼠皮下移植肉瘤的抑制作用及机制探讨[J].山东医药,2015,(30):8-10,3.基金项目
黑龙江省自然科学基金资助项目（201329）；黑龙江中医药大学校基金资助项目（201409）。 （）
