山东医药Issue(31)：8-10,3.DOI:10.3969/j.issn.1002-266X.2015.31.003
LBH589对肾癌细胞株 OS-RC-2增殖的影响及机制探讨
Effect of LBH589 on cell proliferation of renal carcinoma cell line OS-RC-2 and its mechanism
摘要
Abstract
Objective To observe the influences of LBH589 on cell proliferation of renal cancer cell line OS-RC-2 and to explore its mechanism.Methods OS-RC-2 cells were divided into the DMSO group, N-acetyl cysteine ( NAC) group, LBH589 group and NAC+LBH589 group.NAC group was stimulated with 5 mmol/L of NAC for 2 h, LBH489 group was stimulated with 50 nmol/L of LBH589 for 48 h, and the NAC+LBH589 group was pre-treated with 5 mmol/L of NAC for 2 h and then after elution, the group was stimulated with 50 nmol/L of LBH589 for 48 h.EdU assay was used to measure cell proliferation, flow cytometry was used to detect the apoptosis and reactive oxygen species ( ROS) , while Western blotting was used to detect p-STAT3 and Cyclin D1 proteins.Results The proportions of positive Edu cells in the DMSO group, NAC group, LBH589 group and NAC+LBH589 group were respectively 53%±4%, 52%±5%, 7%±3%and 29%±6%.The apoptosis rates of the corresponding groups were 0.25%±0.13%, 0.18%±0.15%, 11.35%±2.40% and 1.92%± 0.63%.ROS of the corresponding groups were 0.60%±0.26%, 0.33%±0.25%, 12.2% ±2.65% and 5.33%± 1.05%.Significant difference was found between the DMSO, NAC+LBH589 groups and the LBH589 group (all P<0.05). The relative expression of p-STAT3 was 1.00 ±0.07, 1.02 ±0.07, 0.49 ±0.04 and 0.52 ±0.05 in the DMSO group, NAC group, LBH589 group and NAC+LBH589 group.The relative expression of Cyclin D1 was 1.00 ±0.08, 1.03 ±0.08, 0.45 ±0.04 and 0.49 ±0.03 in the corresponding groups.Significant difference was found between the DMSO group and the LBH589 group (all P<0.05).Conclusions LBH589 inhibits the growth of renal cancer cell line OS-RC-2, which may be related to induction of apoptosis, inhibition of cell proliferation and the increased ROS level.LBH589 can inhibit STAT3 sig-naling pathway, thus decrease the expression of Cyclin D1.However, LBH589-induced ROS increase is not achieved through the inhibition of STAT3 signaling pathway to inhibit the growth of renal cancer cells.关键词
肾肿瘤/肾癌/细胞增殖/细胞凋亡/组蛋白去乙酰化酶抑制剂Key words
kidney neoplasms/renal cell carcinoma/cell proliferation/apoptosis/histone deacetylase inhibitor分类
医药卫生引用本文复制引用
马俊健,陈婷,刘彬,徐米清..LBH589对肾癌细胞株 OS-RC-2增殖的影响及机制探讨[J].山东医药,2015,(31):8-10,3.基金项目
广东省高等学校优秀青年教师培养计划项目（Yq2013133）。 （）
