色谱Issue(8)：822-829,8.DOI:10.3724/SP.J.1123.2015.03022
超高效液相色谱-四极杆飞行时间质谱法快速筛查水产品中28种酸性合成色素
Rapid screening of 28 acidic artificial dyes in fishery products by ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry
摘要
Abstract
An analytical method was established for the simultaneous determination of 28 acidic artificial dyes in fishery products by ultra performance liquid chromatography coupled with qua-drupole-time of flight mass spectrometry( UPLC-Q-TOF MS). The analytes in fishery products were extracted with ethyl alcohol containing 20%( v/v)ammonia water and 10%( v/v)water. Then the extract solution was purified according to amination adsorbent matrix dispersion method. The compounds were separated by an Eclipse Plus-C 18 column with acetonitrile and 0. 1%(v/v)formic acid-5 mmol/L ammonium acetate solution as the mobile phases,and measured by UPLC-Q-TOF MS with electrospray ionization in negative mode. As a result,the limits of quantification( LOQs,S/N = 10 ) of the target compounds were 20-100 μg/kg in fishery products. The 28 analytes behaved linearly in wide-range with the correlation coeffi-cients more than 0. 991 as well as the relative standard deviations( RSDs)of the peak areas less than 5. 61%( n = 6 ). The average recoveries of the 28 analytes spiked at three levels were ranged from 70. 24% to 106. 47% with RSDs of 5. 62%-9. 65%( n= 6 ). This method can be applied to the analysis of the 28 acidic artificial dyes in fishery products due to its fastness ,sim-plicity and relatively high sensitivity.关键词
超高效液相色谱-四极杆飞行时间质谱/酸性合成色素/水产品/快速筛查Key words
ultra performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOFMS)/acidic artificial dyes/fishery products/rapid screening分类
化学化工引用本文复制引用
王萍亚,周勇,戴意飞,黄鹂,赵巧灵..超高效液相色谱-四极杆飞行时间质谱法快速筛查水产品中28种酸性合成色素[J].色谱,2015,(8):822-829,8.基金项目
国家科技部公益专项（201210060）；舟山市公益类科技项目（2013C31050）． （）
