色谱Issue(8)：849-855,7.DOI:10.3724/SP.J.1123.2015.03030
整体柱在线固相微萃取-高效液相色谱同步富集检测水中的苯氧羧酸类除草剂
Synchronous extraction and determination of phenoxy acid herbicides in water by on-line monolithic solid phase microextraction-high performance liquid chromatography
摘要
Abstract
A C18 monolithic capillary column was utilized as the solid phase microextraction column to construct an in-tube SPME-HPLC system which was used to simultaneously extract and detect five phenoxy acid herbicides,including 2,4-dichlorophenoxyacetic acid(2,4-D),2-(2-chloro)-phenoxy propionic acid(2,2-CPPA),2-(3-chloro)-phenoxy propionic acid(2,3-CPPA),phenoxy propionic acid(PPA)and 2-(2,4-dichlorophenoxy)propionic acid(2,4-DP). The operating parameters of the in-tube SPME-HPLC system ,including the length of the mono-lithic column,the sampling flow rate,the sampling time,the elution flow rate and the elution time,had been investigated in detail. The optimized operating parameters of the in-tube SPME-HPLC system were as follow:the length of the monolithic column was 20 cm,the sampling flow rate was 0. 04 mL/min, sampling time was 13 min;the elution flow rate was 0. 02 mL/min,elution time was 5 min. Under the optimized conditions,the detection limits of the five phenoxy acid herbicides were as follows:9 μg/L for PPA,4 μg/L for 2,2-CPPA,4 μg/L for 2,3-CPPA,5 μg/L for 2,4-D,5 μg/L for 2,4-DP. Compared with the HPLC method with di-rect injection,the combined system showed a good enrichment factors to the analytes. The re-coveries of the five phenoxy acid herbicides were between 79. 0% and 98. 0%( RSD≤3. 9%). This method was successfully used to detect the five phenoxy acid herbicides in water samples with satisfactory results.关键词
在线固相微萃取/高效液相色谱/苯氧羧酸类除草剂/整体柱/同步富集检测Key words
on-line solid phase microextraction/high performance liquid chromatography(HPLC)/phenoxy acid herbicides/monolithic column/synchronous extraction and determination分类
化学化工引用本文复制引用
王家斌,吴芳玲,赵琦..整体柱在线固相微萃取-高效液相色谱同步富集检测水中的苯氧羧酸类除草剂[J].色谱,2015,(8):849-855,7.基金项目
国家自然科学基金青年基金项目（31401609）． （）
