中国组织工程研究2015，Vol.19Issue(30)：4842-4848,7.DOI:10.3969/j.issn.2095-4344.2015.30.017
生物全降解聚左旋乳酸/无定形磷酸钙支架植入大鼠体内后周围组织的钙化
Effect of poly-L-lactic acid/amorphous calcium phosphate scaffold on the surrounding tissue calcification after implantation into the rats
秦超师 1李晓艳 1冯高科 1蒋学俊 1卢钊 1李君1
作者信息
- 1. 武汉大学人民医院心内科,湖北省武汉市 430060
- 折叠
摘要
Abstract
BACKGROUND:Novel fuly biodegradable poly-L-lactic acid/amorphous calcium phosphate (PLLA/ACP) scaffold shows a good prospect of application, but whether the scaffold material has impact on the surrounding tissue calcification is unknown. OBJECTIVE: To observe the influence of PLLA/ACP scaffold material on the calcification of surrounding tissue after implantation of PLLA/ACP scaffold into rats. METHODS:A total of 48 SD rats were divided into experimental group and control group randomly. The experimental group was implanted with PLLA/ACP scaffold material, while the control group was implanted with PLLA scaffold material. At 1, 2, 4, 12 weeks after implantation, the liver function, kidney function and concentrations of calcium, phosphorus, alkaline phosphatase in serum were detected; the muscle tissue around the scaffold was colected for hematoxylin-eosin staining, Von Kossa staining, alkaline phosphatase staining and immunohistochemical staining of nuclear factor-kappa B. Then, western blot assay was used to detect the contents of interleukin-6, bone morphogenetic protein-2, and meanwhile, the contents of calcium and alkaline phosphatase in tissue homogenate were measured. RESULTS AND CONCLUSION:There was no significant difference in either group about the liver and kidney functions at each time. The content of interleukin-6 in the experimental group was less than that in the controlgroup at 2, 4 and 12 weeks after implantation (P < 0.05). The positive expression of nuclear factor-kappa B, bone morphogenetic protein-2 and inflammatory cel count in the experimental group were less than those in the control group at 4 and 12 weeks after implantation (P < 0.05). The content of calcium in the experimental group was less than that in the control group at 12 weeks after implantation (P < 0.05). No difference was found in the expression of alkaline phosphate, the Von Kossa staining and the content of calcium, phosphorus, alkaline phosphatase in the muscle tissue around the scaffold between the two groups (P > 0.05). These findings indicate that the PLLA/ACP scaffold has a good biocompatibility and biological security, which cannot induce peripheral tissue calcification.关键词
生物材料/材料相容性/聚左旋乳酸/无定型磷酸钙/血管支架/SD大鼠/钙化分类
医药卫生引用本文复制引用
秦超师,李晓艳,冯高科,蒋学俊,卢钊,李君..生物全降解聚左旋乳酸/无定形磷酸钙支架植入大鼠体内后周围组织的钙化[J].中国组织工程研究,2015,19(30):4842-4848,7.
