渔业现代化Issue(4)：39-43,5.DOI:10.3969/j.issn.1007-9580.2015.04.009
一株产蛋白酶芽孢杆菌紫外诱变育种的初步研究
Preliminary study on the ultraviolet mutagenesis of a protease producing strain Bacillus Amyloliquefaciens
摘要
Abstract
Aquatic products leftover material after processing are rich in protein, and the leftover material usually,tend to be abandoned.This leads to the protein waste and environmental pollution.In order to utilize this kind of protein, one strain (Hy-743) producing high protease was obtained through mutation of Bacillus Amyloliquefaciens Hy-7 by ultraviolet mutagenesis, The protein hydrolysates had certain antioxidant activity inoculating mutant strain Hy-743 using waste proteins of Spanish mackerel production as substrate. Compared with that of the initial strain , the enzyme activity of the mutant strain increased by 40 .1%, and the total antioxidant activity of the protein hydrolysates increased by 10.2%.The ability to produce enzyme of the mutant strain Hy-743 showed high stability after 8 generations.Using the response surface method to optimize strain Hy-743 for the fermentation conditions, the optimal fermentation conditions are:fermentation temperature 30 ℃, fermentation time for 48 h, initial pH of 6.6, fungus age of 26 h, 2%inoculated quantity, and solid-liquid ratio 1.7 g/50 mL.In clusion, this high yield strain has high protease activity and good genetic stability, and can be used for preparing antioxidant.关键词
蛋白酶产生菌/紫外诱变/蛋白酶活力/抗氧化活性Key words
proteinase strain/ultraviolet mutagenesis/protease activity/antioxidant activity分类
生物科学引用本文复制引用
艾冰花,李秉钧,冯俊荣..一株产蛋白酶芽孢杆菌紫外诱变育种的初步研究[J].渔业现代化,2015,(4):39-43,5.基金项目
山东省科技发展计划项目“水产加工废弃物发酵法制取抗氧化肽的研究（HX12K25）” （）
