中国肺癌杂志Issue(8)：487-492,6.DOI:10.3779/j.issn.1009-3419.2015.08.07
人长链非编码RNA基因SPRY4-IT1对肺癌细胞A549侵袭和迁移能力的影响
Effect of Long Non-coding RNA SPRY4-IT1 on Invasion and Migration of A549 Cells
柴松 1安文婷 1郭丽丽 1姜乐 1高志嵩 1李书军1
作者信息
- 1. 050000石家庄，河北医科大学第二医院肿瘤中心
- 折叠
摘要
Abstract
Background and objective hTe abnormal expression of human long chain non encoding RNA gene is related to many kinds of tumors. hTe aim of this study is to investigate the expression of long non-coding RNA maternally expressed gene 3 (SPRY4-IT1) in lung cancer (A549) cells, and to observe the effect of SPRY4-IT1 on the invasion and migra-tion of A549 cells.Methods hTe levels of SPRY4-IT1 in A549 was detected by Real-time PCR. hTe effects of SPRY4-IT1 on the invasion and migration of A549 cell were analyzed by MTT and Transwell assay. hTe expression of matrix metalloproteinase (MMP) family proteins was determined by Western blot.Results hTe invasion and migration of A549 cells were increased atfer SPRY4-IT1 over-expression. hTe cell spaces were narrower atfer SPRY4-IT1 over expression in the wound healing assay. Tran-swell assays showed that the numbers of transmembrane A549 cells were signiifcantly higher in SPRY4-IT1 over expression group than that in control group (P<0.05). Meanwhile, over expression of SPRY4-IT1 reduced the expression of MMP-2 and MMP-9.Conclusion Over expression of SPRY4-IT1 enhanced the invasion and migration of A549 cells. MMP-2 and MMP-9 might play an important role in this regulation.关键词
肺肿瘤/侵袭/迁移Key words
Lung neoplasms/Invasion/Migration引用本文复制引用
柴松,安文婷,郭丽丽,姜乐,高志嵩,李书军..人长链非编码RNA基因SPRY4-IT1对肺癌细胞A549侵袭和迁移能力的影响[J].中国肺癌杂志,2015,(8):487-492,6.
