中国全科医学Issue(21)：2542-2547,6.DOI:10.3969/j.issn.1007-9572.2015.21.011
硫化氢对高脂诱导的非酒精性脂肪性肝炎大鼠肝脏炎性反应的作用
Impacts of Hydrogen Sulfide on Inflammatory Reaction in High-fat Diet-induced Nonalcoholic Steatosishepatitis Rats
摘要
Abstract
Objective To observe the effect of hydrogen sulfide ( H2 S) on the levels of inflammatory cytokines in liver tissue of rats with nonalcoholic steatosishepatitis (NASH), and to explore the mechanisms of the anti-NASH effect of H2S. Methods 60 male SD rats at SPF level were randomly and equally divided into control group, NASH model group and NaHS intervention group.Rats in control group were fed with normal diet, rats in NASH model group were fed with high fat diet, rats in NaHS intervention group were fed with high fat diet and were injected intraperitoneally with NaHS (56 mg・ kg-1・ d-1 ) , rats in control group and NASH model group were injected intraperitoneally with 0.9% sodium chloride solution.At the end of the 8th week, rats in each group were killed to obtain venous blood and liver tissue samples.The serum level of alanine aminotransferase ( ALT) , aspartate aminotransferase ( AST ) , triglyceride ( TG ) , cholesterol ( TC ) and H2 S, the levels of TG, TC, malondialdehyde ( MDA) , superoxide dismutase ( SOD ) and H2 S in liver tissues were detected by kit.The histopathological changes of the liver tissues were observed under microscope with HE staining.The mRNA and protein expression levels of TNF-αand IL-6 in liver tissues were detected.The protein expression levels of IκBαand NF-κB p65 in the liver tissues were detected.Results The serum levels of ALT, AST, TG, TC, and the levels of TG, TC in hepatic tissues in NASH model group and NaHS intervention group were significantly higher than those in control group respectively, the above indicators in NaHS intervention group were significantly lower than those in NASH model group respectively ( P<0.05) .The levels of H2 S in blood and hepatic tissues in NASH model group were significantly lower than those in NaHS intervention group and control group respectively ( P <0.05 ) .The level of MDA in hepatic tissues in NASH model group and NaHS intervention group was significantly higher than that in control group respectively, whereas the level of SOD in hepatic tissues in NASH model group and NaHS intervention group was significantly lower than that in control group respectively ( P<0.05 ) .The MDA level in NASH model group was significantly lower than that in NaHS intervention group, and the SOD level in NASH model group was significantly higher than that in NaHS intervention group ( P<0.05 ) .Compared with the liver tissues of rats in control group, the liver tissues of rats in NASH model group showed structure disturbance, macrovesicular steatosis, localized necrosis, neutrephil and lymphocytes infiltration.The levels of macrovesicular steatosis, necrosis, and inflammatory reaction in hepatic tissues in NaHS intervention group were significantly lower than those in NASH model group respectively.The mRNA and protein expression levels of TNF-αand IL-6 in liver tissues in NASH model group and NaHS intervention group were significantly higher than those in control group respectively, and the above indicators in NaHS intervention group were significantly lower than those in NASH model group respectively ( P<0.05) .The protein expression level of IκBαin cytoplasm in NaHS intervention group and NASH model group was significantly lower than that in control group respectively, the protein expression level of NF-κB p65 in cell nucleus in NaHS intervention group and NASH model group was significantly higher than that in control group respectively (P<0.05) .The protein expression level of IκBαin cytoplasm in NaHS intervention group was significantly higher than that in NASH model group, the protein expression level of NF-κB p65 in cell nucleus in NaHS intervention group was significantly lower than that in NASH model group ( P <0.05 ) .Conclusion H2 S is effective in improving the pathological changes in hepatic tissues, and it can inhibit inflammatory reaction, which might be one of the mechanisms of the anti-NASH effect of H2 S.关键词
硫化氢/肝炎/炎性反应/大鼠Key words
Hydrogen sulfide/Hepatitis/Inflammatory reaction/Rats分类
医药卫生引用本文复制引用
王丽,王兰辉,孟翠巧,李立萍,宋广昊,任路平,李国风..硫化氢对高脂诱导的非酒精性脂肪性肝炎大鼠肝脏炎性反应的作用[J].中国全科医学,2015,(21):2542-2547,6.基金项目
国家自然科学基金资助项目（81200639）；河北省自然科学基金资助项目 （）
