中国全科医学Issue(21)：2555-2559,5.DOI:10.3969/j.issn.1007-9572.2015.21.013
乌鸡黑色素对1-甲基-4-苯基-1，2，3，6-四氢吡啶致亚急性帕金森病模型小鼠的神经保护作用研究
Neuroprotective Effects of Melanine Derived From Black -bone Silky Fowl on Model Mouse With MPTP -induced Subacute Parkinson′s Disease
摘要
Abstract
Objective To investigate the neuroprotective effects of melanine derived from black -bone silky fowl ( BSF) on model mouse with MPTP-induced Parkinson′s disease ( PD ) and the mechanism.Methods From March 2013 to May 2014, 60 C57/B6 rats were divided into control group (n=10), MPTP group (n=12) and melanine group (n=38) by using random number table; the melanine group was further divided into 10 mg/kg group ( n=13) , 30 mg/kg group ( n=13) and 100 mg/kg group (n=12) .Rats in the control group were administrated with 0.9% sodium chloride solution by gavage;rats in the MPTP group and melanine group were treated with intraperitoneal injection of MPTP (30 mg/kg; one time per day for 7 days); rats in the melanine group were given BSF melanine of different doses ( 10 mg/kg, 30 mg/kg and 100 mg/kg; one time per day for 7 days) by gavage.Rats with unsuccessful model building were excluded, and at last 10 rats in the control group, 10 in the MPTP group, 10 in the 10 mg/kg group, 9 in the 30 mg/kg group and 9 in the 100 mg/kg group were included in the study.Comparison was made among these groups in the expression changes of tyrosine hydroxylase ( TH) -immunoreactive fiber in substantia nigra ( SN) , the number of TH-immunoreactive neurons in the compact part of SN and the protein level of TH corpus striatum.Results In the control group, plenty of TH-immunoreactive fiber was in fasciculation and in a dense and orderly form; in the MPTP model group, TH-immunoreactive nerve fiber was in a sparse, broken and disordered form; in the melanine group, TH -immunoreactive nerve fiber was in fasciculation and in a dense and orderly form, especially in the 30 mg/kg group.The MPTP group, 10 mg/kg group, 30 mg/kg group and 100 mg/kg group were lower ( P<0.05 ) than the control group in the number of TH-immunoreactive neurons in the compact part of SN; the 30 mg/kg group was higher ( P<0.05) than the MPTP group in the number of TH-immunoreactive neurons in the compact part of SN.The MPTP group, 10 mg/kg group, 30 mg/kg group and 100 mg/kg group were lower ( P<0.05) than the control group in the protein level of TH corpus striatum; the 30 mg/kg group and 100 mg/kg group were higher ( P<0.05 ) than the MPTP group in the protein level of TH corpus striatum.Conclusion The intragastric administration of BSF melanine can reduce the death of substantia nigra DA neurons and the loss of corpus striatum TH -immunoreactive nerve fiber in model rats with MPTP -induced subacute PD.The oral administration of BFS melanine has potential neuroprotective effects on PD model rats.关键词
帕金森病/1-甲基-4-苯基-1,2,3,6-四氢吡啶/乌鸡黑色素/小鼠Key words
Parkinson disease/1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine/BSF melanine/Mice分类
医药卫生引用本文复制引用
谢微嫣,王艺铮,王晓民,贺毅..乌鸡黑色素对1-甲基-4-苯基-1，2，3，6-四氢吡啶致亚急性帕金森病模型小鼠的神经保护作用研究[J].中国全科医学,2015,(21):2555-2559,5.基金项目
国家自然科学基金青年科学基金资助项目 （）
