中国全科医学Issue(21)：2584-2588,5.DOI:10.3969/j.issn.1007-9572.2015.21.020
温通活血乳膏对糖尿病周围神经病变大鼠超氧化物歧化酶及丙二醛水平影响研究
Effect of Wen-tong-huo-xue Cream on Levels of Superoxide Dismutase (SOD) and Methane Dicarboxylic Aldehyde (MDA) Among Rats With Diabetic Peripheral Neuropathy
摘要
Abstract
Objective To observe the mechanism of the effect of Wen-tong-huo-xue cream on oxidative stress among rats with diabetic peripheral neuropathy ( DPN) .Methods 50 male SD rats of 8 weeks old were selected as study subjects from May to September in 2013.The rats were divided into normal control group (n=10) and building model group (n=40) by random number table method.10 rats of building model group died during the study, and then the rest rats were equally divided into three groups: model group, lipoic acid group, and Wen-tong-huo-xue cream group by random number table method.Rats in lipoic acid group were injected intraperitoneally with lipoic acid, rats in Wen-tong-huo-xue cream group were bepainted with Wen-tong-huo-xue cream, rats in normal control group and model group were treated with the same amount of matrix.The tail flick latency, latency, motor nerve conduction velocity ( MNCV ) , levels of antioxidant markers〔superoxide dismutase ( SOD ) and methane dicarboxylic aldehyde ( MDA )〕 in serum and sciatic nerve were measured and compared among different groups of rats, the ultra microstructure of sciatic nerve was observed.Results The tail flick latency of rats in model group was significantly longer than that of rats in normal control group ( P<0.05 ); while the tail flick latency of rats in Wen-tong -huo-xue cream group and lipoic acid group was significantly shorter than that of rats in model group, respectively (P<0.05) .The latency of rats in model group was significantly longer than that of rats in normal control group (P<0.05); the latency of rats in Wen-tong-huo-xue cream group was significantly shorter than that of rats in model group (P<0.05) .MNCV of rats in model group, lipoic acid group, and Wen-tong-huo-xue group was significantly shorter than that of rats in normal control group, respectively ( all P<0.05 ); MNCV of rats in lipoic acid group and Wen-tong-huo-xue group was significantly longer than that of rats in model group, respectively ( P<0.05) .Serum level of MDA of rats in model group, lipoic acid group, and Wen-tong-huo-xue group was significantly higher than that of rats in normal control group, respectively ( P<0.05) .There were significant differences in levels of SOD and MDA in sciatic nerve between model group and normal control group ( P<0.05 ) .There was significant difference in sciatic nerve MDA level between lipoic acid group and model group (P<0.05), and there was significant difference in sciatic nerve MDA level between Wen-tong-huo-xue group and model group ( P<0.05 ) .Widened myelin sheath of spinal nerve, damaged lamellar structure, demyelination of nerves, and organelle vesicular degeneration were found among rats in model group; the structural changes of myelin sheath of spinal nerve, lamellar structure, and Schwann cells among rats in Wen -tong -huo -xue cream group and lipoic acid group were slighter than those among rats in model group.Conclusion Wen-tong-huo-xue cream can improve MNCV, alleviate the injury of peripheral nerve structure and function, and its mechanism might be related to adjusting the imbalance between oxidation and reduction, so as to achieve the goal of treating DPN.关键词
糖尿病神经病变/超氧化物歧化酶/丙二醛/温通活血乳膏Key words
Diabetic neuropathies/Superoxide dismutase/Malondialdehyde/Wen-tong-huo-xue cream分类
医药卫生引用本文复制引用
马静,李凯利,马丽..温通活血乳膏对糖尿病周围神经病变大鼠超氧化物歧化酶及丙二醛水平影响研究[J].中国全科医学,2015,(21):2584-2588,5.基金项目
新疆维吾尔自治区自然科学基金资助项目 （）
