中国蔬菜Issue(9):26-30,5.
用于黄瓜白粉病抗性鉴定的InDel标记
InDel Markers for Identification of Cucumber Powdery Mildew Resistance
摘要
Abstract
The co-dominant InDel markers were successfully explored based on the insertion-deletion (InDel)mutation in the major gene/QTL conferring powdery mildew resistance in cucumber(Cucumis sativus L.). The InDel marker InDel-MLO1 was co-segregated with the major gene for powdery mildew resistance through linkage analysis. Known of powdery mildew resistance phenotype,24 cucumber lines originated from various geographical regions of the world were tested by the InDel marker. The results showed that the genotypes of InDel-MLO1 were consistent with their resistance phenotypes in 21 cucumber lines,which indicated that this causal InDel mutation for powdery mildew resistance occurred frequently during the evolution and domestication of cucumber. Therefore,our finding confirmed that the InDel marker is very useful for identification of cucumber powdery mildew resistance and molecular marker-assisted breeding for most of the cucumber lines,and thus,the process of powdery mildew resistance breeding in cucumber will be accelerated.关键词
黄瓜/白粉病/InDel标记/分子标记辅助育种/基因MloKey words
Cucumber/Powdery mildew/InDel marker/Molecular marker-assisted breeding/Gene Mlo引用本文复制引用
聂京涛,李晓丹,姚永康,潘俊松,何欢乐,刘士辉,蔡润..用于黄瓜白粉病抗性鉴定的InDel标记[J].中国蔬菜,2015,(9):26-30,5.基金项目
国家自然科学基金项目(31471892),上海市科委项目(14391900600),上海市浦东新区科技发展基金项目 ()