中国实验动物学报Issue(4)：331-335,5.DOI:10.3969/j.issn.1005-4847.2015.04.001
胆固醇酯转移蛋白转基因家兔制作及生物学特性分析
Establishment of cholesteryl ester transfer protein transgenic rabbits by DNA microinjection and analysis of their biological properties
摘要
Abstract
Objective The aim of this study was to generate human cholesteryl ester transfer protein ( CETP) transgenic rabbits and analyze their biological properties.Methods We generated human CETP transgenic rabbits by DNA microinjection, and detected the expression of human CETP by real-time PCR and Western blot assay.The activity of CETP was measured using an activity assay kit.Results Human CETP transgenic rabbits were successfully generated by DNA microinjection.Compared with wide type rabbits, the expression of human CETP was dramatically increased in the liver of the human CETP transgenic rabbits.The plasma CETP activity was also much higher in the liver of human CETP transgenic rabbits than that of control rabbits.Conclusions The model of human CETP transgenic rabbits is successfully established by DNA microinjection.It will provide a useful tool for the studies of CETP biological function and its involvement in the mechanisms of cardiovascular diseases.关键词
胆固醇酯转移蛋白/转基因/家兔/血浆/肝Key words
Cholesteryl ester transfer protein/Transgene/Rabbits/Plasma/Liver分类
生物科学引用本文复制引用
高守翠,成大欣,赵四海,陈玉龙,王晓靖,白亮,范江霖,刘恩岐..胆固醇酯转移蛋白转基因家兔制作及生物学特性分析[J].中国实验动物学报,2015,(4):331-335,5.基金项目
国家自然科学基金（No．81370379，81070250）。 （）
