中国比较医学杂志Issue(8)：58-61,75,5.DOI:10.3969/j.issn.1671.7856.2015.008.012
软骨细胞特殊染色技术的比较与应用
Comparison of different special staining techniques of chondrocytes and their application values
摘要
Abstract
Objective To compare the advantages and values of several special staining methods of chondrocytes . Methods Twelve 7-day old healthy C57BL/6J mice were killed to obtain the cartilage tissue of the knee joint in order to isolate the chondrycytes .Type II collagen was used to assess the chondrocytes .Then the chondrocyte climbing slices were prepared.The materials were fixed, and HE staining, Safranin O-fast green staining, SA-β-gal staining and immunohistochemical staining of Type II collagen were performed and compared .Results HE staining showed clear morphology of the chondrocytes .The cell nuclei were stained blue and the cytoplasm was pink .Safranin O-fast green staining showed that the nuclei were pink and the cytoplasm green .SA-β-gal staining showed that the aging cells were green while the young cells were colorless .Immunohistochemical staining of type II collagen showed the distribution of type II collagen and they were stained brown while the cell nuclei were blue .Conclusions HE staining and safranin O-fast green staining can provide more information than the other staining techniques .SA-β-gal staining is useful in the analysis of aging chondrocytes .Immunohistochemical of type II collagen can be used to study type II collagen .关键词
软骨细胞/特殊染色/细胞爬片/原代细胞/膝关节Key words
Chondrocytes , special staining/Cell climbing slices/Primary cells/Knee joint, mice分类
医药卫生引用本文复制引用
于斐,曾晖,于红燕,雷鸣,袁昊,肖德明..软骨细胞特殊染色技术的比较与应用[J].中国比较医学杂志,2015,(8):58-61,75,5.基金项目
国家自然科学基金面上项目（项目编号81272032）；深圳市科工贸与信息委员会重点项目（项目编号201101001）；深圳市科创委资助项目（项目编号JCY20130402114702130）。 （）
