中国医学创新Issue(24)：14-15,16,3.DOI:10.3969/j.issn.1674-4985.2015.24.005
动脉留置针在大鼠体脑组织灌注中的应用
In Vivo Perfusion Method of Artery Remaining Needle Applied to Rat Brain Tissue
摘要
Abstract
Objective:To seek a better method for in vivo perfusion of rat brain tissues and improve the efficiency of perfusion fixation.Method:The healthy male SD rat was anesthetized by 10% chloral hydrate,exposed the heart and aortic arch,clipping the thoracic aorta with small arteries clip,with artery remaining needle in the tip of the heart of the rat fluctuation most obviously needled into about 0.5 cm,exited the needle core,at the same time pushed the cannula from the left ventricle to the aorta until the trocar tail,and closed the cannula needle end of the switch to prevent blood spills,then connected with the infusion pipe and cuted the right auricle,fixation brain tissue from the heart perfused sequentially with 0.9%compound sodium chloride injection and 4% paraformaldehyde.Result:The required perfusion fluid of Cerebral tissue perfusion was reduced, perfusion intubation time and total perfusion time was shortened,and the brain tissue after the infusion was harder,the color was more white.Conclusion:By this method of perfusion,the perfusion fixation effect is obviously improved and the operation process is simple and easy to operate,which is worthy of promotion.关键词
动脉留置针/大鼠/脑组织/灌注Key words
Artery remaining needle/Rat/Brain tissue/Perfusion引用本文复制引用
韦晓勇,陆波,闵红星,王毅,李军..动脉留置针在大鼠体脑组织灌注中的应用[J].中国医学创新,2015,(24):14-15,16,3.基金项目
宁夏回族自治区自然科学基金 （）
