中国肿瘤外科杂志Issue(4)：212-216,5.DOI:10.3969/j.issn.1674-4136.2015.04.003
乳腺癌外周血cfDNA ErbB-2启动子区甲基化状态及与肿瘤Her-2表达的关系
cfDNA ErbB-2 promoter methylation status in peripheral blood of non-metastatic breast cancer patients ;and its relationship with Her-2 expression in tumor tissue
梁骁 1郭萌 1宋文哲 1李莹 1祝志强1
作者信息
- 1. 221006 江苏 徐州，徐州医学院附属医院 甲乳外科
- 折叠
摘要
Abstract
Objective To investigate the relationship of methylation of ErbB-2 gene 5 ’ CpG of cfDNA in peripheral blood of non-metastatic breast cancer patients with expression of Her-2 in tumor tissue. Methods I-solate cell-free DNA in peripheral blood of non-metastatic breast cancer patients, analysis methylation of 5’CpG in ErbB-2 gene promoter using bisulfite-sequencing PCR ( BSP) , detect the expression of Her-2 using immuno-histochemistry and FISH. Results Methylation of ErbB-2 gene promoter was only detected in plasma cfDNA of breast cancer patients (19/40), not found in the corresponding peripheral blood cells; Methylation of ErbB-2 gene 5’CpG of cfDNA in peripheral blood of breast cancer patients was negatively correlated with the expression of Her-2 in tumor tissue. Conclusions Methylation of ErbB-2 gene 5’CpG in peripheral blood of breast cancer patients was negatively correlated with the expression of Her-2 in tumor tissue. We can speculate that the methy-lation of ErbB-2 gene 5 ’ CpG in peripheral blood of breast cancer patients can reflect the expression of Her-2 breast cancer tissues to some extent, which may be used as a new method to observe Her-2 status of breast canc-er, and which also can be used to help determine the molecular typing of breast cancer, and used as a new tar-get for breast cancer treatment, and as an early diagnosis indicator of Her-2 positive patients still needs further study.关键词
乳腺癌/BSP/Her-2/ErbB-2/cfDNA/甲基化Key words
breast cancer/BSP/Her-2/ErbB-2/CfDNA/methylation引用本文复制引用
梁骁,郭萌,宋文哲,李莹,祝志强..乳腺癌外周血cfDNA ErbB-2启动子区甲基化状态及与肿瘤Her-2表达的关系[J].中国肿瘤外科杂志,2015,(4):212-216,5.
