工程科学学报Issue(8)：1105-1109,5.DOI:10.13374/j.issn2095-9389.2015.08.020
莱茵衣藻鞭毛内运输蛋白IFT81调控鞭毛组装
Flagellar assembly in Chlamydomonas reinhardtii regulated by intraflagellar transport protein IFT81
摘要
Abstract
A Chlamydomonas reinhardtii immobile mutant named ift81 was screened by insertional mutagenesis. The mutant was bald or with two short flagella. Gene sequencing confirmed that fifty-two base pairs in the fifth extron of IFT81 gene were replaced by the exogenous gene aphⅧ in the mutant. After a recombinant plasmid containing full IFT81 gene was imported into the mutant ift81, the phenotype of the mutant was rescued and the expression of IFT81-HA could be detected, which confirmed that the defect in IFT81 gene led to the abnormal flagella directly. Electron microscopy images exhibited the abnormal flagella of the mutant. An immunofluo-rescence assay revealed that IFT81 protein was localized to the basal body and flagella. This work shows that loss of protein IFT81 will directly lead to the defects of flagellar assembly, and IFT81 protein is indispensable for flagellar assembly in Chlamydomonas reinhardtii.关键词
莱茵衣藻/鞭毛/组装/蛋白Key words
Chlamydomonas reinhardtii/flagella/assembly/protein分类
生物科学引用本文复制引用
孙娟..莱茵衣藻鞭毛内运输蛋白IFT81调控鞭毛组装[J].工程科学学报,2015,(8):1105-1109,5.
