发光学报Issue(9)：1064-1070,7.DOI:10.3788/fgxb20153609.1064
基于金/银纳米三明治结构SERS特性的超灵敏前列腺特异性抗原检测
Hypersensitization Immunoassay of Prostate-specific Antigen Based on SERS of Sandwich-type Au/Ag Nanostructure
摘要
Abstract
Based on surface enhanced Raman scattering (SERS), a super-sensitive immunoassay of prostate specific antigen ( PSA) was developed with the sandwich-type structure consisted of the nano-Ag immune probes and nano-Ag immune substrate. Experimentally, the chemical reduction method was used to prepare Au and Ag nanoparticles, the nano-Au/Ag immune probes were prepared by immobilising Ra-man reporter molecule 4-mercaptobenzoic acid (4-MBA) and anti-prostate specific antigen antibody ( An-ti-PSA) onto the surfaces of Au/Ag nanoparticles. The nano-Ag/Au immune substrates were prepared by in-situ growing of Ag/Au nanoparticles on a silicon wafer and linking with Anti-PSA. The immunoassays of PSA were explored by the four kinds of sandwich-type structures consisted of the nano-Au/Ag immune probes and nano-Au/Ag immune substrates, respectively. It is found that the sandwich-type structure consisted of nano-Ag immune probe and nano-Ag immune substrate is the best one to the immunoassay of PSA, and the detection limit of PSA is as low as 1. 8 fg/mL (3. 490 × 10-18 mol/L), which is suitable to be applied in early detection and diagnosis of prostate cancer.关键词
表面增强拉曼散射/纳米免疫探针/纳米免疫基底/免疫检测Key words
surface-enhanced Raman scattering/nano-immune probe/nano-immune substrate/immunoassay分类
数理科学引用本文复制引用
封昭,周骏,陈栋,王少敏,王小军,谢树森..基于金/银纳米三明治结构SERS特性的超灵敏前列腺特异性抗原检测[J].发光学报,2015,(9):1064-1070,7.基金项目
国家自然科学基金(61275153,61335011,61320106014) （61275153,61335011,61320106014）
浙江省自然科学基金(LY12A04002) （LY12A04002）
宁波大学王宽诚幸福基金资助项目 （）
