分析化学Issue(9)：1272-1277,6.DOI:10.11895/j.issn.0253-3820.150352
功能化Mn掺杂ZnS量子点用于人血清中IgG的低背景磷光检测
Fabrication of IgG Functionalized Mn Doped ZnS Quantum Dots for Low Background Phosphorescent Sensing of IgG in Human Serum
摘要
Abstract
IgG functionalized Mn doped ZnS quantum dots ( IgG-QDs ) were fabricated via the amide condensation between the carboxy groups on the surface of Mn doped ZnS quantum dots and the amino groups on the surface of IgG. The IgG antibody functionalized Au nanoparticals were synthesized via the electrostatic interaction between the Au nanoparticals and IgG antibody. Based on the fluorescence resonance energy transfer effect between the IgG-QDs and the IgG antibody functionalized Au nanoparticals, the IgG-QDs could be applied for low background phosphorescent sensing of IgG with the linear range of 1-10 μg/mL and the limit of detection of 0. 45 μg/mL. The proposed method was successfully applied to detect IgG in human serum without the disturbance of the background fluorescence and scattered light.关键词
Mn掺杂ZnS量子点/免疫球蛋白G/金纳米粒子/磷光Key words
Manganese doped zinc sulfide quantum dots/Immunoglobulin G/Gold nanoparticals/Phosphorescence引用本文复制引用
朱保砚,杨成雄,严秀平..功能化Mn掺杂ZnS量子点用于人血清中IgG的低背景磷光检测[J].分析化学,2015,(9):1272-1277,6.基金项目
本文系国家自然科学基金项目(No.21275079)资助This work was supported by National Science Foundation of China (No.21275079) （No.21275079）
