分析化学Issue(9)：1285-1290,6.DOI:10.11895/j.issn.0253-3820.150369
海水中铁载体的固相萃取预处理和高效液相色谱-串联质谱测定
Determination of Siderophores in Seawater by High Performance Liquid Chromatography-Tandem Mass Spectrometry Coupled with Solid Phase Extraction
摘要
Abstract
Siderophores were produced and secreted with marine microorganisms as the highly specific iron chelators. A method of liquid chromatography-tandem mass spectrometric detection coupled with solid phase extraction pretreatment was developed for the determination of siderophores in seawater. The samples were filtered through a 0. 22 μm membrane, extracted with an ENVI-18 cartridge and then eluted with methanol. The separation of the analytes was performed on a reversed phase SB-C18 column with a gradient elution program by using 0. 1% ( V/V) formic acid and methanol as the mobile phases. Qualitative analysis was performed in multiple-reaction monitoring mode. Good linearity (R2>0. 99) was obtained for Pyoverdines-Fe, Ferrichrome, Ferrioxamine E at the concentrations of 0. 001-3. 00 μg/mL, 0. 005-15. 00 μg/mL, 0. 001-3. 00 μg/mL, respectively. The instrumental detection limits and limits of quantification for the three analytes were 0. 08, 1. 76 and 1. 36 ng/mL; 0. 27, 5. 87 and 4. 53 ng/mL, respectively. The relative standard deviations were lower than 12%, while the recoveries were 12. 1%-18. 6% for Pyoverdines-Fe, 82. 0%-97. 7% for Ferrichrome, and 70. 0%-98. 3% for Ferrioxamine E.关键词
铁载体/固相萃取/高效液相色谱-串联质谱/海水Key words
Siderophore/Analysis/Solid phase extraction/Liquid chromatography-tandem mass spectrometry/Seawater引用本文复制引用
章蕾,袁东星,方锴,刘宝敏..海水中铁载体的固相萃取预处理和高效液相色谱-串联质谱测定[J].分析化学,2015,(9):1285-1290,6.基金项目
本文系国家自然科学基金(No.41176075)资助This work was supported by the National Natural Science Foundation of China (No.41176075) （No.41176075）
