分析化学Issue(9)：1377-1382,6.DOI:10.11895/j.issn.0253-3820.150264
反相超效液相色谱-质谱联用分离分析食用油中的甘油三酯
Separation of Triacylglycerols in Edible Oil by Reversed Phase Ultra Performance Liquid Chromatography-Mass Spectroscopy
摘要
Abstract
A reversed phase ultra performance liquid chromatography-mass spectrometric method was developed for the separation and analysis of triglycerides in edible oils. The samples were separated by using three ultra performance C18 columns in series with a total length of 40 cm (10 cm + 15 cm + 15 cm) at high pressure with acetonitrile-isopropanol (50:50, V/V) as mobile phase at a flow rate of 0. 2 mL/min and at col-umn temperature of 25℃, and detected by APCI ionization-mass spectrometry. The edible oil sample was dis-solved in isopropanol and injected in LC-MS directly. The triglycerides in edible oils were distinguished to their better fine components which included corn oil, peanut oil, sunflower seed oil, rice oil, olive oil, sesa-me oil and soybean oil. The chromatograms of different edible oils showed that the same kind of edible oil was composed of similar triglyceride composition and content, while the different kind of edible oils differed. The experimental result showed that the method could be use for identifying 5% lard adulterated in soybean oil. The method suggests a significant research way for identifying adulteration in edible oil.关键词
反相超效液相色谱-质谱联用/甘油三酯/食用油Key words
Reversed phase ultra performance liquid chromatography-mass spectrometry/Triglycerides/Edible oil引用本文复制引用
何榕,山晓琳,董方圆,许旭..反相超效液相色谱-质谱联用分离分析食用油中的甘油三酯[J].分析化学,2015,(9):1377-1382,6.基金项目
本文系上海市自然科学基金(No.15ZR1440800)资助项目This work was supported by the Natural Science Foundation of Shanghai, China (No.15ZR1440800) （No.15ZR1440800）
