国际生殖健康/计划生育杂志Issue(5)：357-360,363,5.
冷冻保存对人类卵母细胞线粒体DNA缺失和能量代谢的影响
Study on Human Oocyte Mitochondrial DNA Deletions and Energy Metabolism before and after ;Cryopreservation
摘要
Abstract
Objective: To explore the effect of cryopreservation on the mitochondrial DNA (mtDNA) deletions and energy metabolism of human oocyte, so as to investigate the effect of cryopreservation on oocytes preservation. Methods: The dumped oocytes with 0PN, 2PN and 3PN during routine IVF were collected for this cryopreservation study. Those oocytes were randomly divided into two groups, one for vitrification cryopreservation (Group A) and the other as control (Group B, without cryopreservation). The nested polymerase chain reaction (PCR) was used to detect the mtDNA deletions of oocyte mtDNA before and after cryopreservation. Real-time quantitative PCR was adopted to determine the copy number of oocyte mtDNA. ATP contents were detected using luciferin-luciferase assays. Results: There were not significant differences in the mutation frequency of oocyte mtDNA deletions and the copy number of mtDNA between two groups (P>0.05). However, The ATP content in the group A was significantly lower than that in the group B. Conclusions: The effect of cryopreservation on the oocyte mtDNA deletions and the copy number of mtDNA was limited, suggesting that cryopreservation can be properly used to preserve oocyte.关键词
低温保存/卵母细胞/DNA,线粒体/应激,氧化性Key words
Cryopreservation/Oocytes/DNA, mitochondrial/Oxidative stress引用本文复制引用
蔡学泳,彭南妮,刁瑞英,李凌伟,陈燕..冷冻保存对人类卵母细胞线粒体DNA缺失和能量代谢的影响[J].国际生殖健康/计划生育杂志,2015,(5):357-360,363,5.基金项目
深圳市科创委基金（JCYJ20120831112642621）；国家青年科学基金（81401258）；广东省自然科学基金 （）
