河南农业科学Issue(9):91-94,4.DOI:10.15933/j.cnki.1004-3268.2015.09.022
紫外指纹图谱结合 PLS -DA 法鉴定不同产地珠子参
Identification of Panax japonicus var.major from Different Origins Using UV Spectra Fingerprint Combined with Partial Least Squares Discriminant Analysis
摘要
Abstract
To establish a rapid method for the identification of Panax japonicus var.major( Araliaceae) from different geographical origins and provide the basis for quality evaluation,UV spectra combined with partial least squares discriminant analysis( PLS-DA) method was used to distinguish P.japonicus var. major samples from different cultivation regions.Then the difference of P.japonicus var.major in UV spectra was analyzed, and the absorbance was investigated by PLS-DA.The results indicated that the ab-sorption wavelength of the UV fingerprint of P.japonicus var.major samples from different regions was similar, and the peak intensity was different, which could effectively distinguish the P.japonicus var. major samples from different geographical origins.The PLS-DA method successfully separated the samples from Nujiang,Yuxi,Dali and Wenshan.Moreover,samples collected from Dali and Wenshan were similar, while the remaining samples were far from each other.关键词
珠子参/紫外指纹图谱/PLS-DA/产地鉴别Key words
Panax japonicus var.major/UV spectra fingerprint/PLS-DA/identification of origin分类
化学化工引用本文复制引用
钟贵,张霁,张金渝,李涛,王元忠..紫外指纹图谱结合 PLS -DA 法鉴定不同产地珠子参[J].河南农业科学,2015,(9):91-94,4.基金项目
国家自然科学基金(81260610,81260608);科技部“十二五”国家科技支撑计划项目(2011BAI13B01-02,2011BAI13B02-04);农业部公益性行业科研专项(201303117);云南省科技计划项目 ()