湖泊科学Issue(5)：895-901,7.DOI:10.18307/2015.0516
太湖梅梁湾藻蓝蛋白转录间隔区基因和伪空胞基因表达及其影响因子
Environment factors and expression of PC-IGS and gvpC genes in Meiliang Bay of Lake Taihu
摘要
Abstract
The relative expression of intergenic spacer region-within the Phycocyanin( PC-IGS) and gas vesicle gene( gvpC) in cya-nobacteria in Meiliang Bay, Lake Taihu from January of 2013 to January of 2014, was investigated using reverse transcription-quantitative PCR, compared to those in interior Microcystis aeruginosa. In addition, we analyzed the influence of environmental factors on the relative expressions of PC-IGS and gvpC gene. The results showed that the relative expression of PC-IGS increased from January to May of 2013, and reached the maximum in May of 2013. The relative expression of gvpC gene rose from January of 2013, and reached the maximum in March of 2013. The expression of gvpC gene increased earlier than that of PC-IGS. The data also showed that the relative expression of target gene PC-IGS was correlated significantly and positively with the concentrations of nitrate nitrogen, dissolved oxygen, ammonia, nitrite nitrogen and orthophosphate salt, negatively with pH value, and not signifi-cantly with temperature, total nitrogen and total phosphorus. The relative expression of target gene gvpC was correlated significantly and positively with the concentrations of ammonia, nitrate nitrogen and orthophosphate salt. They are positively correlated with the concentrations of total nitrogen and nitrite nitrogen, but negatively with pH value and temperature, and not significantly with the concentrations of dissolved oxygen and total phosphorus in water.关键词
藻蓝蛋白转录间隔区基因/伪空胞基因/反转录定量PCR/环境因子/相对表达量/太湖Key words
PC-IGS/gvpC/reverse transcription-quantitative PCR/environmental factors/relative expression/Lake Taihu引用本文复制引用
张金丽,虞龙,GE Xiuchun,李玉燕,于洋..太湖梅梁湾藻蓝蛋白转录间隔区基因和伪空胞基因表达及其影响因子[J].湖泊科学,2015,(5):895-901,7.基金项目
国家水体污染控制与治理科技重大专项(2012ZX07506-001)和太湖流域(江苏)水生态功能分区与标准管理工程建设课题(2011ZX07534-001)联合资助 （2012ZX07506-001）
