军事医学Issue(8)：610-613,4.DOI:10.7644/j.issn.1674-9960.2015.08.009
miR－873靶向生存蛋白基因对人前列腺癌PC3细胞侵袭性的影响
Impact of miR-873 on human prostate cancer PC3 cells invasion
李锬 1李晨光 1刘岩 1李炳勋 1杨先旭1
作者信息
- 1. 辽宁医学院附属第一医院泌尿外科，辽宁锦州 121001
- 折叠
摘要
Abstract
Objective To identify the role of miR-873, which may regulate the expression of survivin,in human pros-tate cancer PC3 cells invasion.Methods PC3 cells were cultured in vitro, and changes of cellular morphology were ob-served by phase contrast microscope.miR-873,which might regulate the expression of survivin,was predicted by bioinforma-tics and identified using dual luciferase report system.Expressions of miR-873 and survivin were determined using real-time quantitative PCR( qRT-PCR) and Western blotting after transfection of miR-873 mimics.The invasion of PC3 cells was de-tected in vitro by Transwell chamber.Results The expression of survivin was positive by immunofluorescence cytochemis-try.Using dual luciferase reporter system, miR-873 could inhibit the expression of survivin by binding to its mRNA 3′UTR.Results of qRT-PCR and Western blotting showed that overexpression of miR-873 down-regulated the expression of survivin.The invasion of PC3 cells was suppressed by over-expression of miR-873.Conclusion MiR-873 may negatively regulate the expression of survivin in human prostate cancer PC3 cells and inhibit cell invasion.关键词
微小RNA/生存蛋白/前列腺癌PC3细胞Key words
miRNA/survivin/prostate cancer cells PC3分类
医药卫生引用本文复制引用
李锬,李晨光,刘岩,李炳勋,杨先旭..miR－873靶向生存蛋白基因对人前列腺癌PC3细胞侵袭性的影响[J].军事医学,2015,(8):610-613,4.
