林产化学与工业Issue(4)：131-137,7.DOI:10.3969/j.issn.0253-2417.2015.04.021
产香叶醇重组大肠杆菌发酵培养基的优化
Optimization of Medium for Production of Geraniol by the Recombinant Escherichia coli
摘要
Abstract
The Plackett-Burman experiment, the steepest ascent experiment and the central composite design were used to optimize the fermentation medium for the production of geraniol by the recombinant E. coli. Firstly, Plackett-Burman design was used to screen the critical factors which influenced the geraniol production from seven mediums. Secondly, the method of steepest ascent was adopted to make the key factors approach the optimal response area. Finally, the optimal concentration of key factors and regression model were obtained by central composite design. It was indicated that ammonium ferric citrate, MgSO4 , and trace element solution were identified to be the significant factors and the it optimal concentrations were 0. 13 g/L, 2. 12 g/L, and 16. 7 mL/L, respectively. The geraniol concentration increased to 223. 24 mg/L under these conditions. It was about 3. 26 times more than that of before.关键词
香叶醇/培养基优化/响应面/重组大肠杆菌Key words
geraniol/medium optimization/response surface methodology/recombinant E. coli分类
化学化工引用本文复制引用
..产香叶醇重组大肠杆菌发酵培养基的优化[J].林产化学与工业,2015,(4):131-137,7.基金项目
国家863计划资助(2013AA050703-2) （2013AA050703-2）
国家自然科学基金资助项目(21376255) （21376255）
青岛生物能源与过程研究所所长创新基金青年人才专项资助(无编号) （无编号）
