陕西医学杂志Issue(9)：1112-1113,2.DOI:10.3969/j.issn.1000-7377.2015.09.003
右美托咪啶对脂多糖致伤大鼠肾小管上皮细胞TLR4表达的影响﹡
The influence of Toll receptor 4 in renal tubular epithelial cells of dexmedetomidine on rat injured by lipopolysaccharide
摘要
Abstract
Objective :To observe the effect of dexmedetomidine for Toll receptor 4 in renal tubular epi‐thelial cells on rat injured by lipopolysaccharide ,and explore the possible mechanism .Methods :Rat renal tubular epi‐thelial cells and recovery ,training ,then they were added 10 ng/ml lipopolysaccharide(LPS)to culture and divided in‐to A group(blank control group) ,group B(control group) was added 2 .5 μg dexmedetomidine ,group C (experi‐mental group)was joined the 10μg atipamezole ,2 .5 μg dexmedetomidine after 30min ,developing 12h ,then took im‐munohistochemistry to measure expression of TLR4 protein and TLR4 mRNA ,and determine the apoptosis rate of them .Results :Toll receptor 4 level was (45 .1 ± 13 .5) × 103 in group B adopted dexmedetomidine and was lower than those of A and B group ,(tBA = 6 .549 ,tBC = 5 .660 ,P< 0 .05) ,B group’ TLR4mRNA was (0 .46 ± 0 .02)and lower than those of A and C group ,( tBA = 27 .599 ,tBC = 24 .749 ,P< 0 .05) .The apoptosis rate of group B was (34 . 6 ± 7 .6)% and significantly lower than that in A and C group(P < 0 .05) .Conclusion :Dexmedetomidine can down Toll receptor 4 expression in renal tubular epithelial cells of rat injured by lipopolysaccharide ,and the regulation for TLR4 of dexmedetomidine is related with α 2AR excited .关键词
肾疾病/化学诱导/脂多糖类/右美托咪啶/治疗应用/上皮细胞/代谢/动物 ,实验/大鼠/TLR4Key words
Kidney diseases/chemical induced/Lipopolysaccharides/Dexmedetomidine/therapeutic use/Epithelial cells/metabolism/Animals ,laboratory/Rats/TLR4分类
医药卫生引用本文复制引用
雷安锋,段晶,陈燕,彭春晓,王秋月..右美托咪啶对脂多糖致伤大鼠肾小管上皮细胞TLR4表达的影响﹡[J].陕西医学杂志,2015,(9):1112-1113,2.基金项目
辽宁省科技攻关计划项目 （）
