天津科技大学学报2015，Vol.30Issue(4)：25-29,5.DOI:10.13364/j.issn.1672-6510.20140132
氮素对印楝愈伤组织和悬浮细胞培养的影响
Effects of Nitrogen on Azadirachta indica A. Juss Callus and Suspension Culture
摘要
Abstract
To study the effects of nitrogen in MS medium on neem(Azadirachta indica A. Juss)tissue culture,we deter-mined the biomass,soluble protein content,azadirachtin and limonoid contents of neem callus and suspension cells in the end of their cultures by changing the ratio between nitrate and ammonium. The results indicate,that while culturing callus, the four measurement indicators reached the maximum accumulation when the ratio of nitrate to ammonium was 4:1;while culturing suspension cells,the soluble protein contain,azadirachtin and limonoids contains got their maximum whenthe ratio of nitrate to ammonium was 3:1,but the maximum biomass was obtained when the ratio was 2:1. In conclusion,the ratio between nitrate and ammonium in the MS medium should be corresponded with the objects being cultured at different peri-ods.Only in this way,can we get the best target cultures.关键词
印楝/氮素/生物量/可溶性蛋白/印楝素/柠檬苦素Key words
Azadirachta indica A. Juss/nitrogen/biomass/soluble protein/azadirachtin/limonoid分类
农业科技引用本文复制引用
韩广建,李兴林,别振宇,张国运..氮素对印楝愈伤组织和悬浮细胞培养的影响[J].天津科技大学学报,2015,30(4):25-29,5.基金项目
科技部星火计划资助项目(2012GA610011) （2012GA610011）
国家自然科学基金资助项目(U1332123) （U1332123）
