中国组织工程研究Issue(32)：5085-5091,7.DOI:10.3969/j.issn.2095-4344.2015.32.001
三磷酸腺苷对骨髓间充质干细胞成骨和成脂分化的影响
Regulating osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells by extracellular adenosine triphosphate
摘要
Abstract
BACKGROUND:Impaired balance between osteogenesis and adipogenesis of bone marrow mesenchymal stem cels is a crucial pathological mechanism of osteoporosis. Mechanical loads applied to bone tissue can increase bone formation and improve bone strength, and meanwhile lead to the release of extracelular nucleotides, such as adenosine triphosphate. <br> OBJECTIVE: To determine the effects of adenosine triphosphate on the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cels and to investigate the underlying mechanisms. <br> METHODS:The effect of adenosine triphosphate (10, 50, 250 μmol/L) on differentiation of bone marrow mesenchymal stem cels were measured by osteogenic and adipogenic related genes expression, alizarin red staining and oil red O staining. The activation of ERK1/2 signaling pathway by adenosine triphosphate was tested using western blot assay. <br> RESULTS AND CONCLUSION: Incubation of bone marrow mesenchymal stem cels with adenosine triphosphate resulted in the dose-dependent increase of osteogenic genes expression and calcium deposition, and inhibition of adipogenic genes expression and lipid droplet formation, but had no effects on cel proliferation. Adenosine triphosphate activated ERK1/2 signaling pathway, and U0126 as an ERK1/2 inhibitor restrained the effect of adenosine triphosphate on the differentiation of bone marrow mesenchymal stem cels.关键词
干细胞/骨髓干细胞/三磷酸腺苷/骨髓间充质干细胞/成骨分化/成脂分化/ERK信号通路/国家自然科学基金分类
医药卫生引用本文复制引用
李文凯,张英弛,韦盛,杨勇,吴华..三磷酸腺苷对骨髓间充质干细胞成骨和成脂分化的影响[J].中国组织工程研究,2015,(32):5085-5091,7.基金项目
国家自然科学基金青年基金资助项目(81301552)Funding：the National Natural Science Foundation of China, No.81301552 （81301552）
