中国组织工程研究Issue(32)：5210-5214,5.DOI:10.3969/j.issn.2095-4344.2015.32.023
骨髓间充质干细胞联合茶黄素修复激素性股骨头坏死
Bone marrow mesenchymal stem cells combined with theaflavin for steroid-induced avascular necrosis of femoral head
摘要
Abstract
BACKGROUND:Pathological mechanism of femoral head necrosis is not fuly known, and high-dose corticosteroids are prone to induce femoral head necrosis. <br> OBJECTIVE:To investigate the clinical effect of bone marrow mesenchymal stem cels combined with theaflavin on steroid-induced avascular necrosis of femoral head in rats. <br> METHODS: Rat bone marrow mesenchymal stem cels were isolated and culturedin vitro and compounded onto gelatin sponge, and then, divided into four groups: model group, decompression group, cel transplantation group and combined group. Models of steroid-induced avascular necrosis of femoral head were established using liquid nitrogen method. In the combined group, gelatin sponge carrying bone marrow mesenchymal stem cels was transplanted and 250 mg theaflavin was given daily after modeling. <br> RESULTS AND CONCLUSION:At 4 weeks after modeling, the femoral head samples in the four groups were round with exfoliation of articular cartilage that was pale in color. Then, the exfoliation of articular cartilage was aggravated in the model group, and some specimens appeared to colapse; the femoral head specimens in the combined group were round and pale. Until the 8th week after modeling, bone necrosis was increased in the model group; osteoblasts were found in the decompression group with fibrous calus formation; a few of empty bone lacunae appeared with irregular medulary cavity in the cel transplantation group; a large amount of new bone tissues formed in the combined group, and intramedulary adiocytes were in regular array. At 4 and 8 weeks after modeling, the number of empty bone lacunae was significantly lower in the combined group than the other groups (P < 0.05). These findings indicate that bone marrow mesenchymal stem cels combined with theaflavin is an ideal therapy for steroid-induced avascular necrosis of femoral head.关键词
干细胞/移植/茶黄素/骨髓间充质干细胞/激素性股骨头坏死/治疗效果/细胞移植/推广使用/液氮冷冻法/细胞堆积/细胞密度分类
医药卫生引用本文复制引用
赵传勇,丁艳芳,张文志,姚玉龙..骨髓间充质干细胞联合茶黄素修复激素性股骨头坏死[J].中国组织工程研究,2015,(32):5210-5214,5.基金项目
贵州省卫生厅科学技术基金项目(Gzwkj22014-2-1)Funding：the Science and Technology Fund of Guizhou Provincial Health Department, No. Gzwkj22014-2-1 （Gzwkj22014-2-1）
