中国免疫学杂志Issue(9)：1156-1162,7.DOI:10.3969/j.issn.1000-484X.2015.09.002
湖北汉族健康人群肿瘤转移抑制基因 Nm23基因多态性的测定及分布
Distribution of Nm23 gene polymorphism in healthy Chinese Hans population in Hubei,China
摘要
Abstract
Objective:To develop a method for the detection of 10 single nucleotide polymorphisms(SNPs) of Nm23 gene,and to explore the genotypic and allelic distributions of the 10 SNPs in Chinese Hans population in Wuhan.Methods: Two hundreds healthy subjects ,115 men and 85 women included ,were enrolled as DNA sample donors.The real time TaqMan-MGB genotyping assay was used for the determination of the 10 SNPs selected ,and the results were validated by direct gene sequencing.Results:The method established could accurately and quickly screen the genotypes of the 10 SNPs of Nm23/NDPK gene.Distribution frequencies of the 10 SNPs were similar to these in other researches as well as these of HCB.All the loci follow the Hardy-Weinberg equilibrium.Highly linkage disequilibriums were found between rs 16949649 and rs7207370 , rs16949649 and rs34214448 , rs2159359 and rs2302254 , rs2159359 and rs8075231 ,rs2159359 and rs2041296 ,as well as rs2159359 and rs8071647 ,respectively.Four Tag SNPs:rs34214448 , rs2302254 ,rs11868380 and rs2318785 were initially selected by Heploview software.Conclusion:The method established for SNP gen-otyping can meet the needs for rapid analysis of Nm 23 gene polymorphisms ,and may have great values in investigating the association between gene polymorphisms and diseases as well as adverse drug reactions.关键词
Nm23/基因多态性/湖北汉族健康人群Key words
Nm23/NDPK/Single nucleotide polymorphism/Healthy Chinese Hans population分类
医药卫生引用本文复制引用
杨春晓,刘亚妮,沈如飞,周嘉黎,罗小梅,张玉,师少军..湖北汉族健康人群肿瘤转移抑制基因 Nm23基因多态性的测定及分布[J].中国免疫学杂志,2015,(9):1156-1162,7.基金项目
本文受国家自然科学基金项目（No.81273591）、湖北省自然科学基金资助项目（No.2009CDB380）和中央高校基本科研业务费专项资金资助（No.2011JC039）。 （）
